Chromium [Cr(VI)] biosorption property of the newly isolated actinobacterial probiont Streptomyces werraensis LD22

Abstract

The present work demonstrates the heavy metal resistance and detoxification of Cr(VI) by the probiotic actinobacterial cultures isolated from chicken and goat feces. The actinobacterial isolates were screened for heavy metal resistance by qualitative, semiquantitative assays and Cr(VI) biosorption was determined by analytical techniques such as atomic absorption spectrophotometry and Fourier transform infrared spectrometry (FT-IR). All the tested actinobacterial isolates ( n = 20) showed resistance toward K ₂Cr ₂O ₇, NiCl ₂, ZnCl ₂, CuSO ₄ and PbNO ₃ at 20 mg L ⁻¹ concentration. The maximum tolerance concentration values were found to be 200–250 mg L ⁻¹ for K ₂Cr ₂O ₇, 100–250 mg L ⁻¹ for PbNO ₃ and <50–250 mg L ⁻¹ for NiCl ₂, ZnCl ₂ and CuSO ₄. Among the five tested heavy metals, Cr(VI) was resisted by 95 % of the tested actinobacterial cultures up to 250 mg L ⁻¹ concentration; particularly, the isolate LD22 exhibited a high degree of tolerance to all the tested heavy metals. Thus, the isolate was justifiably chosen for Cr(VI) biosorption study and the biosorption efficacy was found maximum at 100 mg L ⁻¹ of metal ion concentration (3 g L ⁻¹ of biomass dosage and pH 7.0). FT-IR spectrum revealed the chemical interactions between the hydroxyl, amine and carboxyl groups of the biomass and the metal ions. On the basis of phenotypic, physiological, biochemical and molecular characteristics the isolate LD22 was identified as Streptomyces werraensis LD22 (JX524481) which could be used to develop a biosorbent for adsorbing Cr(VI) metal ions.

Electronic supplementary material

The online version of this article (doi:10.1007/s13205-014-0237-6) contains supplementary material, which is available to authorized users.