The Effects of Osmosis and Thermo-Priming on Salinity Stress Tolerance in Vigna radiata L.

Abstract

A plant’s response to osmotic stress is a complex phenomenon that causes many abnormal symptoms due to limitations in growth and development or even the loss of yield. The current research aimed to analyze the agronomical, physiological, and biochemical mechanisms accompanying the acquisition of salt resistance in the Vigna radiata L. variety ‘Ramzan’ using seed osmo- and thermopriming in the presence of PEG-4000 and 4 °C under induced salinity stresses of 100 and 150 mM NaCl. Seeds were collected from CCRI, Nowshera, and sowing was undertaken in triplicate at the Department of Botany, Peshawar University, during the 2018–2019 growing season. Rhizospheric soil pH (6.0), E.C (2.41 ds/m), field capacity, and moisture content level were estimated in the present study. We observed from the estimated results that the agronomic characteristics, i.e., shoot fresh weight and shoot dry weight in T9 (4oC + 150 mM NaCl), root fresh weight and root dry weight in T4 (PEG + 100 mM NaCl), shoot moisture content in T5 (PEG + 100 mM NaCl), and root moisture content in T6 (PEG + 150 mM NaCl) were the highest, followed by the lowest in T1 (both shoot and root fresh weights) and T2 (shoot and root dry weights). Similarly, the shoot moisture content was the maximum in T5 and the minimum in T6, and root moisture was the highest in T6. We observed from the estimated results that agronomical parameters including dry masses (T4, T6, T4), leaf area index, germination index, leaf area, total biomass, seed vigor index under treatment T9, and relative water content and water use efficiency during T5 and T6 were the highest. Plant physiological traits such as proline, SOD enhanced by T1, carotenoids in treatment T2, and chlorophyll and protein levels were the highest under treatment T4, whereas sugar and POD were highest under treatments T7 and T8. The principal component analysis enclosed 63.75% of the total variation among all biological components. These estimated results confirmed the positive resistance by Vigna radiata during osmopriming (PEG) and thermopriming (4 °C) on most of the features with great tolerance under a low-saline treatment such as T4 (PEG), T5 (PEG + 100 mM NaCl), T7 (4 °C), and T8 (4 °C + 100 mM NaCl), while it was susceptible in the case of T6 (PEG + 150 mM NaCl) and T9 (4 °C + 150 mM NaCl) to high salt application. We found that the constraining impact of several priming techniques improved low salinity, which was regarded as economically inexpensive and initiated numerous metabolic processes in plants, hence decreasing germination time. The current study will have major applications for combatting the salinity problem induced by climate change in Pakistan.