CyTOF protocol for immune monitoring of solid tumors from mouse models

Summary

Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects.

For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021). ¹

Graphical abstract

Highlights

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  Facilitating discovery through high-parameter immune profiling in tumor mouse models

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  Design surface and intracellular antibody panels specific to experimental needs

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  Immune and non-immune live-cell barcoding scheme to enable multiplexing of samples

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  Mass cytometry (cytometry by time-of-flight; CyTOF) for mouse tumors and whole blood

Abstract

Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Abstract

Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects.