Molecular detection and identification of Wolbachia in three species of the genus Lutzomyia on the Colombian Caribbean coast

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Abstract

Background

The hematophagous habits of insects belonging to the genus Lutzomyia (Diptera: Psychodidae), as well as their role as biological vectors of Leishmania species, make their presence an indication of infection risk. In the present study, seven species of Lutzomyia were identified and screened for natural infections with Wolbachia.

Methods

Collection of sand flies was done in an endemic focus of leishmaniasis on the Colombian Caribbean coast (Department of Sucre, Ovejas municipality). DNA collected from Lutzomyia species was evaluated with PCR for wsp gene amplification to screen for bacterial infection.

Results

Endosymbiotic Wolbachia was found in three species: Lutzomyia c. cayennensis, Lutzomyia dubitans and Lutzomyia evansi. Two Wolbachia strains (genotypes) were found in Lutzomyia spp. These genotypes were previously unknown in dipteran insects. The wLev strain was found in Lutzomyia dubitans, L. c. cayennensis and L. evansi and the wLcy strain was found only in L. c. cayennensis.

Conclusions

Genetic analysis indicated that the Wolbachia strains wLcy and wLev belong to the B Supergroup. This study provides evidence of infections of more than one strain of Wolbachia in L. c. cayennensis.

Electronic supplementary material

The online version of this article (doi:10.1186/s13071-017-2031-x) contains supplementary material, which is available to authorized users.

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Most cited references 23

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Precision Farming: Technologies and Information as Risk-Reduction Tools

Franklin Hall (1999)

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Multilocus sequence typing system for the endosymbiont Wolbachia pipientis.

Laura Baldo, Julie Dunning Hotopp, Keith Jolley … (2006)

The eubacterial genus Wolbachia comprises one of the most abundant groups of obligate intracellular bacteria, and it has a host range that spans the phyla Arthropoda and Nematoda. Here we developed a multilocus sequence typing (MLST) scheme as a universal genotyping tool for Wolbachia. Internal fragments of five ubiquitous genes (gatB, coxA, hcpA, fbpA, and ftsZ) were chosen, and primers that amplified across the major Wolbachia supergroups found in arthropods, as well as other divergent lineages, were designed. A supplemental typing system using the hypervariable regions of the Wolbachia surface protein (WSP) was also developed. Thirty-seven strains belonging to supergroups A, B, D, and F obtained from singly infected hosts were characterized by using MLST and WSP. The number of alleles per MLST locus ranged from 25 to 31, and the average levels of genetic diversity among alleles were 6.5% to 9.2%. A total of 35 unique allelic profiles were found. The results confirmed that there is a high level of recombination in chromosomal genes. MLST was shown to be effective for detecting diversity among strains within a single host species, as well as for identifying closely related strains found in different arthropod hosts. Identical or similar allelic profiles were obtained for strains harbored by different insect species and causing distinct reproductive phenotypes. Strains with similar WSP sequences can have very different MLST allelic profiles and vice versa, indicating the importance of the MLST approach for strain identification. The MLST system provides a universal and unambiguous tool for strain typing, population genetics, and molecular evolutionary studies. The central database for storing and organizing Wolbachia bacterial and host information can be accessed at http://pubmlst.org/wolbachia/.

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The genetics and cell biology of Wolbachia-host interactions.

Frédéric Landmann, Laura Serbus, William Sullivan … (2007)

Wolbachia are gram-negative bacteria that are widespread in nature, carried by the majority of insect species as well as some mites, crustaceans, and filarial nematodes. Wolbachia can range from parasitic to symbiotic, depending upon the interaction with the host species. The success of Wolbachia is attributed to efficient maternal transmission and manipulations of host reproduction that favor infected females, such as sperm-egg cytoplasmic incompatibility (CI). Much remains unknown about the mechanistic basis for Wolbachia-host interactions. Here we summarize the current understanding of Wolbachia interaction with insect hosts, with a focus on Drosophila. The areas of discussion include Wolbachia transmission in oogenesis, Wolbachia distribution in spermatogenesis, induction and rescue of the CI phenotype, Wolbachia genomics, and Wolbachia-membrane interactions.

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Author and article information

Contributors

Rafael José Vivero: rajovigo2001@yahoo.com

Gloria Cadavid-Restrepo: gecadavi@unal.edu.co

Claudia Ximena Moreno Herrera: cxmoreno@unal.edu.co

Sandra I. Uribe Soto: suribe@unal.edu.co

Journal

Journal ID (nlm-ta): Parasit Vectors

Journal ID (iso-abbrev): Parasit Vectors

Title: Parasites & Vectors

Publisher: BioMed Central (London )

ISSN (Electronic): 1756-3305

Publication date (Electronic): 28 February 2017

Publication date PMC-release: 28 February 2017

Publication date Collection: 2017

Volume: 10

Electronic Location Identifier: 110

Affiliations

[1 ]Universidad Nacional de Colombia at Medellín, Medellín, Colombia

[2 ]Grupo de Investigación en Sistematica Molecular, Universidad Nacional de Colombia at Medellín, Medellín, Colombia

[3 ]ISNI 0000 0000 8882 5269, GRID grid.412881.6, PECET (Programa de Estudio y Control de Enfermedades Tropicales), , Universidad de Antioquia, ; Medellín, Colombia

[4 ]Grupo de Microbiodiversidad y Bioprospección, Laboratorio de Biología Celular y Molecular, Universidad Nacional de Colombia at Medellín, Medellín, Colombia

Article

Publisher ID: 2031

DOI: 10.1186/s13071-017-2031-x

PMC ID: 5329942

PubMed ID: 28241782

SO-VID: 7a3381b9-f4e5-4eee-a3a0-49c908c3f12a

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 16 July 2016

Date accepted : 10 February 2017

Funding

Funded by: Departamento Administrativo de Ciencia, Tecnología e Innovación (CO)

Award ID: code # 357-2011 U.T EICOLEISH - “Comprehensive Strategy for the Control of Leishmaniasis

Funded by: FundRef http://dx.doi.org/10.13039/100007637, Departamento Administrativo de Ciencia, Tecnología e Innovación;

Award ID: National Call 528 for doctoral studies in Colombia, 2011

Custom metadata

ScienceOpen disciplines: Parasitology

Keywords: wolbachia,phylogroup wleva,wsp gene,lutzomyia,natural infection

Data availability:

ScienceOpen disciplines: Parasitology

Keywords: wolbachia, phylogroup wleva, wsp gene, lutzomyia, natural infection

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Molecular detection and identification of Wolbachia in three species of the genus Lutzomyia on the Colombian Caribbean coast

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Abstract

Background

Methods

Results

Conclusions

Electronic supplementary material

Related collections

Malaria vaccine development

Most cited references 23

Precision Farming: Technologies and Information as Risk-Reduction Tools

Multilocus sequence typing system for the endosymbiont Wolbachia pipientis.

The genetics and cell biology of Wolbachia-host interactions.

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Cited by 9

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