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      Effect of Planting Distance and Dosage of organic fertilization on the Growth and Productivity Stove Purple Flower Orthosiphon stamineus Benth. Stems

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          Abstract

          Medicinal plant cultivation is essentially a way of management so that a medicinal plant can bring high yields and good quality. This condition can occur if the plant can grow in a suitable environment, including commensurate soil fertility, and suitable climate with appropriate technology. One of the plants that can be developed vegetatively is the Orthosiphon stamineus Benth.s, which is very useful as a kidney medicine, for epilepsy, hepatitis, and gallstones. Orthosiphon stamineus Benth.s plant extract can also be used for anti-fungal, anti-bacterial, anti-allergic, anti-hypertensive, and anti-inflammatory purposes. The purpose of the study was to determine the effect of planting distance and dose of organic fertilization on the growth and production of stove purple flower Orthosiphon stamineus Benth.s. The research took place from July to October 2020 at the Experimental Garden of B2TP Lampung. Materials Orthosiphon stamineus Benth. stem cuttings, organic fertilizer (cow manure), water. Tools used were a hand tractor, Cultivator, hoe, scissors, meter, vernier, plastic basket, tray, and oven. This study used a Split plot Design with three replications. The treatment variables used were planting distance as the main plot consisting of 3 levels (0.4 mx0.4 m; 0.5 m x 0.4 m; and 0.6 m x 0.4 m). Then the dose of organic fertilizer as a subplot consisting of 4 levels (150 grams per plant, 300 grams per plant, 450 grams per plant, and 600 grams per plant). The experiment was repeated 3 times so that there were 36 experimental plots. The results of the analysis of variance for the production of Orthosiphon stamineus Benth. s. yields, there is an interaction of planting distance treatment and organic fertilizer dosing that gives significantly different results (p<0.01) for the parameters of wet production per plant, wet productivity per hectare, dry production per plant, and dry productivity per hectare. While the results of the analysis of variance for plant growth, the number of branches is influenced by planting distance and organic fertilizer application. A planting distance of 0.6 x 0.4 m and 300 grams of manure is the optimal treatment to increase the growth and productivity of Orthosiphon stamineus Benth. plants.

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          Regulation of plant biomass production.

          Plant biomass used for cellulosic biofuel production is primarily from vegetative tissues. Therefore, an increase in the vegetative growth of plants will lead to a higher production of plant biomass. Recent molecular and genetic studies have identified a number of factors that regulate processes such as vegetative meristem activities, cell elongation, photosynthetic efficiency, and secondary wall biosynthesis, all of which are crucial for plant biomass production. The regulators of these processes could potentially be applied to improve the yield of biomass crops such as Miscanthus, switchgrass, and hybrid poplar in the near future. Copyright 2010 Elsevier Ltd. All rights reserved.
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            Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism

            Background In the present study, we tested a 50% ethanolic extract of Orthosiphon stamineus plants and its isolated bioactive compound with respect to their α-glucosidase and α-amylase inhibitory activities. Methods Bioactive flavonoid sinensetin was isolated from 50% ethanolic extract of Orthosiphon stamineus. The structure of this pure compound was determined on the NMR data and the α-glucosidase and α-amylase inhibitory activities of isolated sinensetin and 50% ethanolic extract of Orthosiphon stamineus were evaluated. Results In vitro studies of a 50% ethanolic extract of O. stamineus and the isolated sinensetin compound showed inhibitory activity on α-glucosidase (IC50: 4.63 and 0.66 mg/ml, respectively) and α-amylase (IC50: 36.70 mg/ml and 1.13 mg/ml, respectively). Inhibition of these enzymes provides a strong biochemical basis for the management of type 2 diabetes via the control of glucose absorption. Conclusion Alpha-glucosidase and α-amylase inhibition could the mechanisms through which the 50% ethanolic extract of O. stamineus and sinensetin exert their antidiabetic activity, indicating that it could have potential use in the management of non-insulin-dependent diabetes.
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              Constituents of the Vietnamese medicinal plant Orthosiphon stamineus.

              From the MeOH extract of the aerial part of Vietnamese Orthosiphon stamineus, five new isopimarane-type diterpenes [orthosiphols F-J (1-5)] and two new diterpenes [staminols A (6) and B (7)] with a novel carbon-framework, to which we proposed the name "staminane", and three new highly-oxygenated staminane-type diterpenes [staminolactones A (8) and B (9) and norstaminol A (10)1 were isolated. Moreover, staminolactone A (8) is 8,14-secostaminane-type and staminolactone B (9) is 13,14-secostaminane-type, while norstaminol A (10) is 14-norstaminen-type. Together with these new diterpenes, sixteen known compounds were also isolated and identified to be: 7,3',4'-tri-O-methylluteolin (11), eupatorin (12), sinensetin (13), 5-hydroxy-6,7,3',4'-tetramethoxyflavone (14), salvigenin (15), ladanein (16), tetramethylscutellarein (17), 6-hydroxy-5,7,4'-trimethoxyflavone (18), vomifoliol (19), aurantiamide acetate (20), rosmarinic acid (21), caffeic acid (22), oleanolic acid (23), ursolic acid (24), betulinic acid (25), and beta-sitosterol (26). All the isolated compounds were tested for their cytotoxicity towards highly liver metastatic murine colon 26-L5 carcinoma cells, and the new diterpenes, except for 4, and flavonoids (11, 12, 16, 18) showed cytotoxicity with an ED50 value between 10 and 90 microg/ml.
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                Author and article information

                Journal
                BIO Web of Conferences
                BIO Web Conf.
                EDP Sciences
                2117-4458
                2023
                October 16 2023
                2023
                : 69
                : 01024
                Article
                10.1051/bioconf/20236901024
                10506fde-3869-4815-a15d-725dd568d60a
                © 2023

                https://creativecommons.org/licenses/by/4.0/

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