Bacterial resistance to antibiotic alternatives: a wolf in sheep’s clothing? ¹

Abstract

Implications Substantial pressure to reduce antibiotic use has necessitated the development of antibiotic alternatives. However, relatively little consideration has been given to the development of resistance to these alternatives. Whether we come up with antibiotic alternatives that are bacteriocidal or inhibitory, bacteria will continue to adapt and evolve. Some antibiotic alternatives support the development of antibiotic resistance necessitating caution. There are opportunities to optimize antibiotic alternative effectiveness as well as to minimize the development of resistance mechanisms. Introduction With the growing concern of antibiotic resistance (Aminov and Mackie, 2007; Zaman et al., 2017), there has been a strong push to reduce the use of antibiotics in animal production systems (Van Boeckel et al., 2015; Ventola, 2015). Many antibiotic alternatives have been developed, with varying degrees of success in improving health outcomes and growth performance (Gresse et al., 2017). These alternatives use very different approaches to regulate both commensal and pathogenic bacterial populations. Antibiotic alternatives such as phage and bacteriocins have very clear mechanisms of antimicrobial activity (Figure 1), whereas others, such as essential oils/phytosterols, have less defined modes of action. Irrespective of mode of action, there has been insufficient attention given to the ability of bacteria to develop resistance to these antibiotic alternatives. Considering the development of resistance will be essential in finding long-term solutions. In this review, we present what is known about the ability of bacteria to become resistant to these antibiotic alternatives, and more importantly, identify where they contribute to antibiotic resistance. Prudence is required, as avoiding further contribution to antibiotic resistance is necessary. This review is not exhaustive but is intended to give a good representation from different classes of antibiotic alternatives. In particular, we focus on phage, essential oils, direct-fed microbials and bacteriocins, metals and minerals, and organic acids. Some consideration is given to their application, effectiveness, and modes of action. Figure 1. (A) Phage interact with specific receptors to inject DNA into the bacterial cell, causing viral proliferation and cell lysi (i). Essential oils (EOs) disrupt efflux/influx, membrane receptors and stability (ii). Copper disrupts bacterial lipids, proteins, and DNA through oxidization (iii). Bacteriocins cause cell wall lysis, disrupt the plasma membrane structure (pore formation), and interfere with DNA function (iv). (B) Bacterial resistance to phage is conferred through either blockage/removal of the receptor or cutting of phage DNA in the cell by CRISPR/CAS (i). Bacteria form aggregates to minimize cell surface exposure to EOs, thus preventing membrane associated disruptions (ii). Glutathione chelates Cu+, ATPase efflux system exports Cu+/Cu2+, and siderophores sequester Cu2+ to prevent it entering the cell (iii). Modifications of the cell wall and membrane affect fluidity and charge, impairing bacteriocin binding (iv). Bacteriophages Bacteriophages are viruses that can infect and kill bacteria. In the environment, there is a constant arms race between bacteria and phages: as bacteria develop resistance mechanisms, new phages emerge. Bacteriophages are highly specific, which makes them intriguing antibiotic alternatives as they are less likely to affect commensal bacteria. Although, bacteriophages have not yet been widely adopted in animal production systems, they have been shown to be effective in controlling pathogenic bacteria in feed animals. Research has shown potential for phages to control colonization of Campylobacter jejuni (Carrillo et al., 2005), and Salmonella (Borie et al., 2008; Bardina et al., 2012), while decreasing mortality in chickens during Escherichia coli infection (Huff et al., 2002; Huff et al., 2006). Phages have also been effective in reducing Salmonella shedding in pigs (Saez et al., 2011) and reducing shedding of E. coli O157:H7 in sheep (Bach et al., 2009; Raya et al., 2011). However, efforts targeting E. coli O157 in cattle have proven to be less successful (Rozema et al., 2009; Rivas et al., 2010; Stanford et al., 2010). While phages present potential for the control of pathogenic bacteria, there are significant considerations still required regarding their implementation in animal production. As with antibiotics, bacteria are capable of developing resistance to phage infection utilizing systems such as the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) system as a pseudoimmune system, or the abortive infection system to kill infected cells before the phage can spread (Labrie et al., 2010). Additionally, bacteria can alter their cell surface to remove or block the receptor to which the phage binds (Labrie et al., 2010), which can impact virulence or colonization factors (Cryz et al., 1984). For example, the occurrence of phage-resistant C. jejuni has been noted; however, all isolates exhibited decreased ability to colonize the cecum of broiler chickens (Carrillo et al., 2005). As resistance to multiple phages can be difficult for bacteria to develop, the use of phage cocktails which target different receptors is recommended. This has been shown to result in superior reduction of bacterial cells with fewer incidence of resistant strains and is commonly used in studies examining phage treatment (Huff et al., 2002; Carrillo et al., 2005; Rivas et al., 2010). Another factor to consider in the use of phages is the characteristics of the phages themselves. Phages can be both lytic and lysogenic in nature, with only lytic phages being appropriate for phage treatment. This is due to the fact that lysogenic phages do not always result in lytic infection, leaving some bacteria alive with the phage genome inserted into their own. Additionally, lysogenic phages are capable of contributing to the transfer of antibiotic resistance and virulence genes across bacterial populations (Wagner and Waldor, 2002; Balcazar, 2014). Because of these factors, in addition to utilizing cocktails of phages to prevent the development of resistance, it is important that all phages to be used for treatment or prophylaxis in animal production be thoroughly tested to ensure purely lytic infections can occur with their use. Antibacterial Metals, Minerals, and Nanoparticles Unlike phage, metals including Copper (Cu2+/Cu+), Zinc (Zn2+), and Silver (Ag+), and nonmetal elements, such as Iodine (I2), have been used in animal production for their broad-spectrum antibacterial activity and low generation of resistance (Aarestrup and Hasman, 2004; Murdoch and Lagan, 2013; Wang et al., 2016). Copper, zinc, and silver disrupt bacterial protein functions, generate reactive oxygen species, and cause damage to bacterial DNA (Rosen, 2002; Xiu et al., 2014). Although iodine’s antimicrobial activity is not well understood, there is indication that it works by reacting with unsaturated fatty acids in the lipid bilayer of the cell wall to cause leaks, as well as inactivating nuclear materials through coagulation (Murdoch and Lagan, 2013). As a result of their broad-spectrum antimicrobial activity, it was believed that generation of bacterial resistance to Cu2+, Zn2+, Ag+, and I2 should be low (Martínez-Abad et al., 2012; Murdoch and Lagan, 2013; Wang et al., 2016). Copper and Zinc salts are commonly added to animal feeds in concentrations above dietary requirements because of their antimicrobial activity, which results in reduced infection and improved animal growth. Similarly, Zinc Oxide added to pig diets has been effective in reducing post-weaning diarrhea (Mazaheri Nezhad Fard et al., 2011; Pieper et al., 2012; Holman and Chénier, 2015; Vahjen et al., 2015). Additionally, copper has been determined to be an effective antimicrobial for udder washes, proving active against a panel of bacteria and yeasts associated with bovine mastitis (Reyes-Jara et al., 2016). The antimicrobial properties of copper and zinc when added to feed have created a selective pressure for bacteria that contain resistance to these heavy metals (Mazaheri Nezhad Fard et al., 2011). High inclusion rates alter the gut microbiome, however, many bacteria have developed resistance, with both zinc- and copper-resistant enterococci identified from the gut microbiome of pigs (Mazaheri Nezhad Fard et al., 2011; Pieper et al., 2012; Vahjen et al., 2015). Bacterial resistance genes to zinc and copper are located on mobile genetic elements, often plasmids, which are transferable between bacteria (Aarestrup and Hasman, 2004; Mazaheri Nezhad Fard et al., 2011; Richard et al., 2017). More importantly, bacteria resistant to copper and zinc have indicated increased resistance to antibiotics, as an increased dose of dietary zinc oxide in weaned pigs increased tetracycline and sulfonamide resistance genes (Mazaheri Nezhad Fard et al., 2011; Vahjen et al., 2015). This increase in resistance is likely due to mechanisms of cross-resistance or coresistance: when microbes use the same resistance mechanism to defend against different antimicrobials such as an efflux pump, or when the genes responsible for resistance are linked closely and are transcribed or transferred together (El Behiry et al., 2012; Vahjen et al., 2015; Reyes-Jara et al., 2016). The genes associated with resistance to copper and zinc have been found on the same plasmids that contain antibiotic resistance genes, and the selective pressure of these metals can result in the sharing of antibiotic resistance among bacteria (Mazaheri Nezhad Fard et al., 2011; Yin et al., 2017), as depicted in Figure 2. Figure 2. The selective pressure of copper results in the uptake of foreign plasmids by enterococci, conferring copper resistance genes as well as antibiotic resistance genes (coresistance). Silver and iodine have been used for many years as antimicrobial agents for wounds and external infections because of their broad-spectrum activity against bacteria and low development of resistance (Martínez-Abad et al., 2012; Murdoch and Lagan, 2013; Kalan et al., 2017). Iodine and silver are active antimicrobial ingredients used in both human and animal wound care products (Burks, 1998; Murdoch and Lagan, 2013; Kalan et al., 2017), and iodine has commonly been used as an udder wash (Tremblay et al., 2014). Extracellular polymeric substances found in biofilms contain functional groups capable of binding metal ions, like silver, and protect against antimicrobial agents such as iodine (Kang et al., 2014; Tremblay et al., 2014; Xiu et al., 2014). Other defenses include mechanisms against oxidative stress, protein/DNA damage repair mechanisms, and metal efflux pumps (Gupta et al., 1999; Tremblay et al., 2014; Xiu et al., 2014). However, studies have shown that silver is capable of penetrating and killing biofilms (Heidari Zare et al., 2017; Kalan et al., 2017; Lemire et al., 2017) and multidrug-resistant pathogens (Kalan et al., 2017). Recent research has indicated that silver resistance is due to the Sil operon that resides on plasmid pMG101 identified in Salmonella enterica serovar Typhimurium, which when transferred to E. coli, conferred silver resistance (Woods et al., 2009; Asiani et al., 2016). Plasmid pMG101 also contains resistance genes to a list of antibiotics, including ampicillin, chloramphenicol, tetracycline, streptomycin, and sulphonamide, suggesting that the transfer of pMG101 between bacteria under the selective pressure of silver may also result in sharing of other antibiotic resistance (Woods et al., 2009). However, incidence of silver resistance remains low (Woods et al., 2009; Kalan et al., 2017) which may indicate that plasmid pMG101 is restricted to particular species or is difficult to transfer or be maintained by other bacteria (Woods et al., 2009). Up until 2013, no known generation of bacterial resistance to iodine had been identified, and studies looking at repeated iodine use over time did not indicate any increase in resistant bacteria (Murdoch and Lagan, 2013). Mastitis-associated bacteria have shown to produce biofilms to survive treatment with low concentrations of iodine (Tremblay et al., 2014). Using sublethal concentrations of nonoxinol-9 iodine complex on Staphylococcus aureus strains specific to mastitis resulted in the development of resistance, although the mechanisms of tolerance are unknown (El Behiry et al., 2012). Although other research has indicated cross-resistance of antibiotics with other biocides (El Behiry et al., 2012), currently there is no known cross-resistance with iodine and antibiotics (El Behiry et al., 2012; Murdoch and Lagan, 2013). Given the challenges with feeding high dose metals, in particular, bacterial resistance and environmental effects of run-off, metal nanoparticles have gained attention as an alternative (Yin et al., 2017). Metal nanoparticles such as silver, copper oxide, and zinc oxide are of particular interest for their antimicrobial properties and suitability as feed additives (Beyth et al., 2015). Technological advances have decreased the cost of synthesizing nanoparticles and made their inclusion in livestock diets more feasible in recent years (Fondevila et al., 2009). The mode of action for antimicrobial metal nanoparticles is not completely elucidated; potential mechanisms include cell membrane disruption, generation of reactive oxygen species, and disruption of protein structure (Beyth et al., 2015). The increased surface area to volume ratio of smaller particles, as well as properties such as shape, can all contribute to an increased bactericidal activity compared to their corresponding metal ions (Gautam and Van Veggel, 2013; Rudramurthy et al., 2016). Zinc oxide nanoparticles have been demonstrated as effective bactericidal agents against antibiotic resistant S. aureus and Staphylococcus epidermidis (Ansari et al., 2012). Silver nanoparticles have also been shown to be effective against bacterial and fungal species, including some important pathogens (Rudramurthy et al., 2016). While in many respects metal nanoparticles may be a promising tool, use of this technology could also generate bacterial resistance. Strain-specific minimum inhibitory concentrations of nanoparticles in E. coli and S. aureus have already been identified, demonstrating that varied resistance to nanoparticle antimicrobial mechanisms exist naturally in the bacterial population (Ruparelia et al., 2008). There is also the risk of accumulation of nanoparticles in livestock tissues particularly if these products are used over long time periods, and the implications for animal health and food safety are not yet completely understood (Fondevila et al., 2009; De Jong et al., 2013; Adeyemi and Faniyan, 2014). Prior to use, it will be necessary to determine if interaction between a specific nanoparticle and biological tissues results in undesired degradation by-products, inflammation, or oxidative stress (Gautam and Van Veggel, 2013; Rudramurthy et al., 2016). Metal nanoparticles may be able to confer similar or improved benefits as antibiotic alternatives in livestock; however, more work needs to be done to fully understand their antimicrobial mechanisms, and impacts on tissues and the environment before they can be readily used in livestock production systems. Organic Acids Organic acids have been used in the food industry as preservatives and disinfectants for many years, and more recently have gained interest as feed additives for livestock (Ricke, 2003; Upadhaya et al., 2014). Some organic acids that have been tested include formic, acetic, sorbic, fumaric, lactic, propionic, citric, and benzoic acid. The proposed mode of action for organic acids involves conversion into their antibacterial forms in the gastrointestinal tract and diffusion into bacterial cells, decreasing cell internal pH (Ricke, 2003; Bearson et al., 1997). Providing a mixture of organic acids to finisher pigs or broiler chicks has been shown to decrease E. coli counts and increase growth performance (Mohamed et al., 2014; Upadhaya et al., 2014). Similarly, the addition of formic or propionic acid in a Salmonella infection model decreased cecal Salmonella counts in chicks at 7, 14, and 21 days of age (McHan and Shotts, 2015). These effects on common pathogens suggest that organic acids could be a promising alternative to antibiotics; however, results are contradictory. In a different experiment, formic acid inclusion resulted in no effect on average daily gain of broiler chickens compared to a control diet, though changes in intestinal morphology were observed (Garcia et al., 2007). It is still unclear whether organic acids can improve growth performance and animal health across different livestock production settings, or if their efficacy is reliant on certain external factors. In stored feed the concentration of organic acids required to decrease pathogens depends on feed composition and pathogen status; for example, bacteria in stationary phase may be more resistant (Ricke, 2003). In vivo, their efficacy depends in part on factors such as pH, where low pH allows more undissociated acid to remain intact and functional (Baik et al., 1996). Protection via encapsulation may also improve efficacy by supplying acids to the intestine in their undissociated form and preventing absorption or metabolism before the products reach their desired location (Upadhaya et al., 2014). Perhaps the most concerning drawback to the use of organic acids is their ability to induce acid tolerance responses in exposed bacteria. This tolerance response can result in the ability to withstand short-term exposure to pH as low as 3 (Baik et al., 1996). Over one generation, bacteria can increase tolerance to more extreme acid conditions (Ricke, 2003; Bearson et al., 1997). Bacterial species, such as Salmonella, naturally encounter low pH as well as short-chain fatty acids as part of their transit of the gastrointestinal tract, and can cope with these stressors using RNA polymerase, sigma S dependant systems (Baik et al., 1996). This acid stress response can increase pathogen survival in the stomach or in phagosomes, leading to increased virulence in both Salmonella and pathogenic E. coli (Ricke, 2003; Bearson et al., 1997). Acid tolerance has also been shown to increase shedding of E. coli O157:H7 in calves and mice (Price et al., 2000). Acid adaptation can also improve bacterial resistance to heat, salt, and H2O2, which could have serious implications for food safety and preservation (Bearson et al., 1997). Increased resistance to these other stressors may also lead to bacterial resistance to other antimicrobial alternatives such as metal ions or nanoparticles, further decreasing the number of tools available to help maintain animal health. Essential Oils Extracted oils from the roots, seeds, leaves, bark, flowers, and fruits of plants contain complex mixtures of phenolic compounds known for their antimicrobial, anti-inflammatory, and antioxidants activities (Ayseli and Ipek Ayseli, 2016). The bioactive components in essential oils can modify both bacterial and host cellular functions by interacting with cell wall components and lipid membranes, which in the right conditions can lead to cell death (Omonijo et al., 2017). A major concern, as with antibiotics, is that over time bacteria may adapt and become resistant to the active phenolic components. Studies have evaluated the antibacterial activity of essential oils and purified phytogenic compounds on both pathogenic and gut commensal bacteria. Pathogenic bacteria are sensitive to an array of essential oils at concentrations ranging from 0.02 to 0.7 g/L (Yang et al., 2015). Antimicrobial activity was assessed in 28 different essential oils by disk diffusion method on pathogenic S. enterica and on beneficial Lactobacillus plantarum. From the evaluation, essential oils from oranges had the best selective antibacterial activity against pathogenic bacteria with diminished activity on beneficial species (Ambrosio et al., 2017). Essential oils have been shown to inhibit multidrug-resistant bacteria independently of their antibiotic resistance profile (Becerril et al., 2012). Antifungal properties have also been documented against multiple strains of Candida albicans isolated from cattle with mastitis. Rosemary terpenes are suggested to act together on C. albicans by disrupting cellular integrity, respiration, ion transport, and membrane permeability (Ksouri et al., 2017). Similarly, citrus essential oils are suspected to disrupt the cell membrane due to the less abundant compounds working synergistically rather than one dominant compound such as thymol and carvacrol found in select herbal essential oils (Ambrosio et al., 2017). In this light, the antimicrobial mode of action of essential oils may be specific to one compound or the result of many. The effectiveness of essential oils against common pathogens, E. coli O157:H7 and S. enterica, is dependent on the concentration of active phenolic compounds (Friedman et al., 2017). A cause of concern comes from an outbreak of S. enterica infections traced back to contaminated basil leaves. The subinhibitory concentrations of basil permitted S. enterica to develop resistance to its active component linalool (Kisluk et al., 2013). Bacterial resistance mechanisms toward essential oils include selective membrane permeability, regulated efflux/influx and chemotaxis-controlled motility (Kalily et al., 2016). Linalool-associated adaptations, although protective in vitro, may have a significant fitness cost on the adapted bacteria in a challenging environment (Kalily et al., 2017). Bacteria-associated adaptations complicate the use of essential oils and long-term studies are needed to understand whether the adapted resistance is a transmissible function. Phytogenic compounds have good potential as an alternative to antibiotics in animal production, both as growth promoters and as treatment for bacterial infections (Omonijo et al., 2017; Reyer et al., 2017). Concentrations of the active components must be tested in vivo to determine whether an effective dose can be reasonably achieved. Additionally, their lipophilic nature may limit delivery to enteric pathogens, but again, microencapsulation for targeted release can help (Yang et al., 2015). A new strategy to combine essential oils with either disruptive metals, antibiotics, and/or nanotechnologies has gained attention to effectively combat multiresistant strains of bacteria and reduce bacterial resistance (Kwiatkowski et al., 2017; Low et al., 2017; Omonijo et al., 2017). The synergistic effect of phenolic compounds in combination with other environmental challenging applications may be a safer and more effective approach to address growing concerns of bacterial resistance. Microbial Approaches Direct-fed microbials, or probiotics, have been evaluated as alternatives to antimicrobial growth promoters in livestock production. The effectiveness of direct-fed microbials as growth promoters and therapeutic antimicrobials is highly variable (McAllister et al., 2011), but positive effects, such as improving feed efficiency, weight gain, nutrient digestibility, intestinal morphology, and reducing potential pathogens and diarrhea occurrence have been reported in livestock species (Salim et al., 2013; Hou et al., 2015; Lei et al., 2015; Uyeno et al., 2015). The strains selected for use as probiotics must be evaluated for the presence of antimicrobial resistance genes that could potentially be transferred to pathogenic bacteria in the gut. Antibiotic susceptibility in 46 Lactobacillus strains obtained from the human gut and dairy products was evaluated by disc-diffusion method, and all strains had shown resistance to a group of 14 antibiotics (Charteris et al., 1998). One strain of Lactobacillus reuteri, a probiotic candidate that can reduce E. coli and Salmonella pullorum growth in vitro, showed resistance to several antibiotics, including tetracycline, chloramphenicol, vancomycin, streptomycin, bacitracin, and penicillin G (Zhang et al., 2012). Identification and removal of resistance determinants, creating mutants with similar probiotic capacities, can potentially be used as a strategy to overcome the risk of horizontal gene transfer from probiotics to pathogens (Rosander et al., 2008). Several direct-fed microbial strains produce short-chain fatty acids, which can reduce gut pH and inhibit pathogen growth (Kamada et al., 2013). However, in vitro studies found that Listeria monocytogenes can develop tolerability to acidic conditions, which is associated with increased resistance to other stressors and increased virulence (O’Driscoll et al., 1996; Conte et al., 2000), and as discussed above. A promising microbial approach that might replace or complement antibiotic treatments is the use of purified bacteriocins or bacteriocin-producing microorganisms (Cavera et al., 2015). Bacteriocins are peptides ribosomally synthesized by bacteria and archaea (Riley and Wertz, 2002; Cotter et al., 2005) that vary in size, structure, mechanism of action, antimicrobial potency, immunity mechanisms, target cell receptors (Gillor et al., 2008), and bactericidal spectrum (Cotter et al., 2005). Bacteriocins can facilitate the dominance of a producer in a competitive environment (Dawid et al., 2007), regulate gut microbiota (Bhardwaj et al., 2010), and inhibit pathogen growth, without affecting others members of the microbial community (Dabour et al., 2009). The mechanisms by which bacteriocins exert their bactericidal and bacteriostatic effects include cell wall and plasma membrane disruption, impairment of protein synthesis, interference with DNA replication and transcription, and induction of cell autolysis (Cavera et al., 2015; Ahmad et al., 2017). Bacteriocins can be used to treat infectious diseases such as mastitis caused by Streptococcus dysgalactiae in lactating cows (Ryan et al., 1998), to preserve food products, and to promote the establishment of probiotic strains (Cotter et al., 2005; Cotter et al., 2013; Martinez et al., 2016). However, innate and/or acquired resistance to bacteriocins are frequently reported (Ahmad et al., 2017). Identified mechanisms of bacteriocin resistance in Gram positive bacteria include cell wall modifications and alteration of the cell membrane composition, which affect membrane fluidity and electrical charges, therefore impairing bacteriocin ability to bind to bacterial cells. These mechanisms are similar to those of resistance to antibiotics, and this similarity raises concern regarding the development of cross-resistance (Zhou et al., 2014). Bacteriocin-resistant L. monocytogenes downregulate the expression of mannose phosphotransferase system, impairing the binding of bacteriocins to the cell membrane; but this resistance is accompanied by a reduction in pathogen growth rate compared to the sensitive strain (Masias et al., 2017), indicating that the development of resistance to bacteriocins may increase energy costs and compromise fitness of resistant strains (Martinez et al., 2016). Some bacteriocins have been engineered for improved efficacy and stability (Cavera et al., 2015), and their use as therapeutic agents is a rapidly developing area of research (Lohans and Vederas, 2012). However, there are currently only a few commercially bacteriocin-based products available for veterinary use (Martinez et al., 2016). Conclusion It is clear that there is substantial effort going into the development of antibiotic alternatives to support healthy and efficient animal production. As we move forward with these technologies, it is important to keep resistance mechanisms in mind so that these technologies can be sustained. Most importantly, some of these antibiotic alternatives, such as zinc oxide, can clearly contribute to increased antibiotic resistance and should therefore be avoided. For other antibiotic alternatives, such as phage and bacteriocins, the potential contribution to antibiotic resistance is less clear, but should be considered. The idea of using more specific antibiotic alternative therapies, such as bacteriophage and bacteriocins, is enticing, as they do not affect commensal microbes. Furthermore, new phage will always be available as a result of the constant arms race between bacteria and bacteriophage. Ultimately, any strategy used must be economically viable, but we must avoid complacency and ensure that we are not replacing a wolf (antibiotic resistance) with a wolf in sheep’s clothing (Figure 3). Figure 3. Antibiotic alternatives may be a danger in disguise. Pig image: many antimicrobial alternatives target the early post-weaning piglet. Polyphenols: polyphenolic compounds found in plant products, such as these pea seed coats, have strong antibacterial activity.