Sulfur-doped titanium dioxide exhibits a strong visible-light-induced antibacterial effect. The new photocatalyst can effectively kill Micrococcus lylae, a common Gram-positive bacterium. The relationship between sulfur concentration and the bactericidal activity of S-doped TiO2 was investigated. Results from DMPO spin-trapping electron spin resonance measurements confirm the formation of hydroxyl radicals, which is the origin of the considerable bactericidal activity under visible light irradiation.