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      Antibiofilm and antimicrobial activity of Lactobacillus cell free supernatant against Pseudomonas aeruginosa isolated from burn wounds

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          Abstract

          The present study investigated the antimicrobial and anti‐biofilm effects of indigenous Lactobacillus probiotic strains on Pseudomonas aeruginosa isolated from burn wound infection in laboratory conditions. The effect of 7 probiotic strains isolated from infant faeces on the pathogenicity factors of P. aeruginosa, including protease, elastase, antibiofilm and antipyocyanin was measured. Also, diffusion methods in the well and micro broth dilution were used to evaluate the antimicrobial activity of probiotics. All tests were performed in triplicate. A negative control and a positive control were used for each test. SPSS version 22 software was used for statistical analysis, and a p < 0.05 was considered statistically significant. A total of 30 clinical isolates of P. aeruginosa were isolated. The elastolytic activity of P. aeruginosa isolates decreased after adding Cell free supernatant (CFS) of each Lactobacillus. L1, L4, L5, and L6 strains had a 100% inhibitory effect on pathogen isolates. L3 and L7 strains had the lowest inhibitory effect. The inhibitory effect of CFS extracted from lactobacilli on protease production by P. aeruginosa. L1, L4, L5, and L6 strains had an inhibitory effect on all tested isolates. L2, L3, and L7 strains had a less inhibitory effect. L4 strain had the highest inhibitory effect on pyocyanin production by P. aeruginosa (50%), followed by L5 (43.3%), L1 (40%), and L6 (23.3%) strains. L3 and L7 strains had no inhibitory effect on the pyocyanin production of P. aeruginosa isolates. It was found that the CFS of 4 isolates (L1, L4, L5, and L6) was the most active extract and had a 100% inhibitory effect against biofilm formation of all P. aeruginosa strains. The L3 strain had the least inhibitory effect against the biofilm formation of pathogens. Overall, this study showed that probiotics could be promising alternatives to combat the pathogenicity of P. aeruginosa in burn wounds.

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          Interference of Lactobacillus plantarum with Pseudomonas aeruginosa in vitro and in infected burns: the potential use of probiotics in wound treatment.

          B C Valdez, Lakhoua Rachid, Perla M. Santana … (2005)
          This study evaluated the ability of the probiotic organism Lactobacillus plantarum to inhibit the pathogenic activity of Pseudomonas aeruginosa, both in vitro and in vivo, and investigated the mechanisms involved in such protection. L. plantarum whole cultures, culture filtrates (acid filtrate and neutralised acid filtrate) and isolated, washed cells were tested in vitro for their effects on the production of the P. aeruginosa quorum-sensing signal molecules, acyl-homoserine-lactones (AHLs), and two virulence factors controlled by these signal molecules, elastase and biofilm. All were inhibited by L. plantarum cultures and filtrates, but not by isolated, washed cells. The acid L. plantarum growth medium itself had some inhibitory activity, but the greatest activity was exerted by the whole culture. To test the in-vivo activity of L. plantarum, a burned-mouse model was used in which burns infected with P. aeruginosa were treated with L. plantarum at 3, 4, 5, 7 and 9 days post-infection. Samples from skin, liver and spleen taken after 5, 10 and 15 days demonstrated inhibition of P. aeruginosa colonisation by L. plantarum. There was also an improvement in tissue repair, enhanced phagocytosis of P. aeruginosa by tissue phagocytes, and a decrease in apoptosis at 10 days. These results indicate that L. plantarum and/or its by-products are potential therapeutic agents for the local treatment of P. aeruginosa burn infections.
          Article 0 comments Cited 82 times – based on 0 reviews     Review now
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            Antibiotic resistance in Pseudomonas aeruginosa and alternative therapeutic options.

            Maitrayee Chatterjee, C Anju, Lalitha Biswas … (2016)
            Pseudomonas aeruginosa is a leading cause of nosocomial infections and is responsible for ∼10% of all hospital-acquired infections worldwide. It continues to pose a therapeutic challenge because of the high rate of morbidity and mortality associated with it and the possibility of development of drug resistance during therapy. Standard antibiotic regimes against P. aeruginosa are increasingly becoming ineffective due to the rise in drug resistance. With the scope for developing new antibiotics being limited, alternative treatment options are gaining more and more attention. A number of recent studies reported complementary and alternative treatment options to combat P. aeruginosa infections. Quorum sensing inhibitors, phages, probiotics, anti-microbial peptides, vaccine antigens and antimicrobial nanoparticles have the potential to act against drug resistant strains. Unfortunately, most studies considering alternative treatment options are still confined in the pre-clinical stages, although some of these findings have tremendous potential to be turned into valuable therapeutics. This review is intended to raise awareness of several novel approaches that can be considered further for combating drug resistant P. aeruginosa infections.
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              Probiotics to counteract biofilm-associated infections: promising and conflicting data

              Claudia Vuotto, Francesca Longo, Gianfranco Donelli (2014)
              Altered bowel flora is currently thought to play a role in a variety of disease conditions, and the use of Bifidobacterium spp. and Lactobacillus spp. as probiotics has been demonstrated to be health-promoting, even if the success of their administration depends on the applied bacterial strain(s) and the targeted disease. In the last few decades, specific probiotics have been shown to be effective in the treatment or the prevention of acute viral gastroenteritis, pediatric post-antibiotic-associated diarrhea, some pediatric allergic disorders, necrotizing enterocolitis in preterm infants, inflammatory bowel diseases and postsurgical pouchitis. The potential application of probiotics is continuously widening, with new evidence accumulating to support their effect on the prevention and treatment of other disease conditions, including several oral diseases, such as dental caries, periodontal diseases and oral malodor, as well as genitourinary and wound infections. Considering the increasingly widespread ability of pathogens to generate persistent biofilm-related infections, an even more attractive proposal is to administer probiotics to prevent or counteract biofilm development. The response of biofilm-based oral, intestinal, vaginal and wound infections to probiotics treatment will be reviewed here in light of the most recent results obtained in this field.
              Article 0 comments Cited 55 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Hadi Sedigh Ebrahim Saraei: seddigh.hadi@gmail.com
                Meysam Hasannejad‐Bibalan: meysam.hasannejad@gmail.com
                Journal
                Journal ID (nlm-ta): Int Wound J
                Journal ID (iso-abbrev): Int Wound J
                Journal ID (doi): 10.1111/(ISSN)1742-481X
                Journal ID (publisher-id): IWJ
                Title: International Wound Journal
                Publisher: Blackwell Publishing Ltd (Oxford, UK )
                ISSN (Print): 1742-4801
                ISSN (Electronic): 1742-481X
                Publication date (Electronic): 16 July 2023
                Publication date Collection: December 2023
                Volume: 20
                Issue: 10 ( doiID: 10.1111/iwj.v20.10 )
                Pages: 4112-4121
                Affiliations
                [ 1 ] Student Research Committee, School of Medicine Guilan University of Medical Sciences Rasht Iran
                [ 2 ] Farhikhtegan Medical Convergence Sciences Research Center,Farhikhtegan Hospital Tehran Medical Sciences Islamic AzadUniversity Tehran Iran
                [ 3 ] Burn and Regenerative Medicine Research Center Guilan University of Medical Sciences Rasht Iran
                [ 4 ] Department of Microbiology, School of Medicine Guilan University of Medical Sciences Rasht Iran
                [ 5 ] Department of Biostatistics and Epidemiology, School of Health Guilan University of Medical Sciences Rasht Iran
                Author notes
                [*] [* ] Correspondence

                Hadi Sedigh Ebrahim Saraei, Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

                Email: seddigh.hadi@ 123456gmail.com

                Meysam Hasannejad‐Bibalan, Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

                Email: meysam.hasannejad@ 123456gmail.com

                Article
                Publisher ID: IWJ14305
                DOI: 10.1111/iwj.14305
                PMC ID: 10681627
                PubMed ID: 37455022
                SO-VID: e96c3c43-0ab0-4129-acae-7b8c94eaf8a7
                Copyright © © 2023 The Authors. International Wound Journal published by Medicalhelplines.com Inc and John Wiley & Sons Ltd.
                License:

                This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

                History
                Date revision received : 19 June 2023
                Date received : 03 June 2023
                Date accepted : 22 June 2023
                Page count
                Figures: 2, Tables: 1, Pages: 10, Words: 7075
                Categories
                Subject: Original Article
                Subject: Original Articles
                Custom metadata
                source-schema-version-number 2.0
                cover-date December 2023
                details-of-publishers-convertor Converter:WILEY_ML3GV2_TO_JATSPMC version:6.3.5 mode:remove_FC converted:27.11.2023

                ScienceOpen disciplines: Emergency medicine & Trauma
                Keywords: burns,lactobacillus,probiotics,pseudomonas aeruginosa,wounds and injuries
                Data availability:
                ScienceOpen disciplines: Emergency medicine & Trauma
                Keywords: burns, lactobacillus, probiotics, pseudomonas aeruginosa, wounds and injuries

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