Comparison of Freestyle Optium Neo H and Centrivet GK Device in the Diagnosis of Hypoglycaemia and Hyperketonaemia in Dairy Goats: A Field Study

ABSTRACT

Background

There is a lack of data on the validation and diagnostic performance of the Freestyle Optium Neo‐H (Freestyle) and Centrivet GK (Centrivet) devices for the diagnosis of hypoglycaemia, hyperglycaemia and hyperketonaemia in goats.

Objectives

The aim of the present study was to validate the Freestyle and Centrivet for the analysis of whole blood beta‐hydroxybutyric acid (BHBA) and to validate the Freestyle for the analysis of whole blood glucose concentrations using the reference method (RM) in goat blood collected from the jugular and ear veins.

Methods

Venous blood samples were utilised to assess glucose and BHBA concentrations using the Freestyle, Centrivet and RM. The cut‐off point of BHBA was ≥ 0.8 mmol/L for hyperketonaemia. A total of 198 paired blood samples (vena jugularis and ear vein) were collected from 99 hair goats. The cut‐off point for hypoglycaemia diagnosis was < 49 mg/dL.

Results

There were proportional but no constant errors between RM and Freestyle and Centrivet for BHBA, and both proportional and constant errors were observed for glucose analysis. The mean bias for BHBA analysis was 0.14 and 0.06 mmol/L (Freestyle‐RM) 0.51 and 0.16 mmol/L (Centrivet‐RM) for jugular and ear veins, respectively. The mean bias for blood glucose analysis was 0.0 and 5.6 mg/L between Freestyle and RM in the jugular and ear veins, respectively. The sensitivity (Centrivet: 50%–61.3%; Freestyle: 93.6%–75.8%) and specificity (Centrivet GK: 75.7%–73%; Freestyle: 37.8%–70.3%) were determined in jugular and ear vein blood for hyperketonaemia diagnostics, respectively. The AUC of Freestyle was 0.89 and 0.95 in the jugular and ear vein for hypoglycaemia, respectively. The sensitivity of Freestyle was 60.3% and 96.8% in the jugular and ear vein for hypoglycaemia. The specificity of Freestyle was 100.0% and 76.7% for hypoglycaemia in jugular and ear veins, respectively.

Conclusions

Freestyle demonstrated acceptable diagnostic performance for hypoglycaemia in ear veins, but neither Freestyle nor Centrivet showed sufficient diagnostic performance for hyperketonaemia. Both analysers were not interchangeable with RM in BHBA and glucose analysis.

Abstract

In this study, the diagnostic performance of Freestyle Optium Neo‐H and Centrivet GK devices in hyperketonaemia and hypoglycaemia or hyperglycaemia goats was evaluated. Freestyle demonstrated acceptable diagnostic performance for hypoglycaemia in ear veins, but neither Freestyle nor Centrivet showed sufficient diagnostic performance for hyperketonaemia. The findings suggest a need for future studies focused on the development of Freestyle Optium Neo‐H and Centrivet GK chips and test kits specifically tailored for goats.