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Abstract
The exact role of neutrophils in the pathogenesis of TB is poorly understood. Recent
evidence suggests that neutrophils are not simply scavenging phagocytes in Mycobacterium
tuberculosis (Mtb) infection.
Three different types of clinical specimens from patients with active pulmonary TB
who underwent lung surgery were examined: sputum, BAL fluid, and cavity contents.
Differential cell separation and quantification were performed for intracellular and
extracellular bacteria, and bacterial length was measured using microscopy.
Neutrophils were more abundant than macrophages in sputum (86.6% +/- 2.2% vs 8.4%
+/- 1.3%) and in BAL fluid (78.8% +/- 5.8% vs 11.8% +/- 4.1%). Inside the cavity,
lymphocytes (41.3% +/- 11.2%) were the most abundant cell type, followed by neutrophils
(38.8% +/- 9.4%) and macrophages (19.5% +/- 7.5%). More intracellular bacilli were
found in neutrophils than macrophages in sputum (67.6% +/- 5.6% vs 25.2% +/- 6.5%),
in BAL fluid (65.1% +/- 14.4% vs 28.3% +/- 11.6%), and in cavities (61.8% +/- 13.3%
vs 23.9% +/- 9.3%). The lengths of Mtb were shortest in cavities (1.9+/- 0.1 microm),
followed by in sputum (2.9 +/- 0.1 microm) and in BAL fluid (3.6 +/- 0.2 microm).
Our results show that neutrophils are the predominant cell types infected with Mtb
in patients with TB and that these intracellular bacteria appear to replicate rapidly.
These results are consistent with a role for neutrophils in providing a permissive
site for a final burst of active replication of the bacilli prior to transmission.