Cryo-Microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunofluorescence reaction (IIF) was 94.4% and 92.5% and the specificity 94.2% and 97.1% for the two specific larval antigens, respectively. Sera from 123 persons (54 from carriers of S. stercoralis infections and 69 from controls) were submitted to the reaction. The titers of different sera varied from 20 to 2560. There was a significant linear correlation (r = 0.85 p <FONT FACE="Symbol">£</font> 0.001) between the antibodies from the two species of larval antigens. We conclude that both antigens may be used in the IIF reaction for the diagnosis of human strongyloidiasis. Due to the feasibility of safe and low-cost mass production of S. ratti larvae in the laboratory with a considerable economy of conjugate, their utilization in the serum diagnosis of human strongyloidiasis is recommended

Translated abstract

Comparou-se o emprego de cortes em congelação de larvas de S. stercoralis e de S. ratti como fonte de antígenos na reação de imunofluorescência indireta (RIF) para o diagnóstico da estrongiloidíase humana. Os antígenos foram obtidos de coproculturas de fezes humanas e de ratos, respectivamente. Soros de 123 indivíduos foram analisados sendo 54 de pacientes com estrongiloidíase e 69 controles. Empregou-se conjugado anti IgG humano marcado com fluoresceína nos títulos ideais de 10 para S. stercoralis e 100 para S. ratti. A sensibilidade da RIF foi de 94,4% e 92,5% e a especificidade de 94,2% e 97,1% respectivamente para os antígenos de S. stercoralis e S. ratti. Para os 2 antígenos os títulos dos soros variaram de 20 a 2560. Houve correlação linear significativa (r = 0,85 p <FONT FACE="Symbol">£</font> 0,001) entre os títulos de anticorpos para os 2 antígenos. Concluiu-se que os 2 antígenos podem ser empregados na RIF. Diante da disponibilidade de obtenção de grande quantidade de antígeno em laboratório, economia do conjugado, por não haver diferença significativa entre sensibilidades e especificidades e haver correlação linear entre os títulos de anticorpos, a utilização do S. ratti é recomendada como antígeno no diagnóstico da estrongiloidíase humana

Related collections

Most cited references 36

Record: found
Abstract: found
Article: not found

Global prevalence of strongyloidiasis: critical review with epidemiologic insights into the prevention of disseminated disease.

R M Genta (2016)

Opportunistic disseminated strongyloidiasis is an important cause of morbidity and mortality in immunocompromised patients. However, the worldwide prevalence of Strongyloides stercoralis is difficult to determine because adequate data are lacking. In this paper more than 100 epidemiologic studies reporting on the prevalence of S. stercoralis among various populations on five continents are critically reviewed. Analysis of this information indicates that the following groups of people may be at risk: residents of an emigrants from any developing country and southern, eastern, and central Europe; travelers and veterans returning from endemic areas; natives and residents of the Appalachian region in the United States and local endemic areas in other countries; and institutionalized persons. Because disseminated infections may be prevented by early treatment of asymptomatic chronic infections, screening programs are recommended to detect latent S. stercoralis infection before the initiation of chemotherapy or immunosuppression in patients at risk. Serologic tests are sensitive and specific but are not yet widely available. Thus strong suspicion based on clinical and epidemiologic clues in conjunction with repeated stool examinations remains the best way to rule out S. stercoralis infection in these groups.