Effect of bacteria treated and untreated post-methanated distillery effluent (PMDE) on seed germination, seedling growth and amylase activity in Phaseolus mungo L
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Abstract
Present study deals the effect of bacteria treated and untreated post-methanated distillery
effluent (PMDE) on germination, seedling growth and amylase activity in Phaseolus
mungo L. seeds. Results revealed that untreated PMDE was highly toxic in nature carrying
high BOD, COD values along with high metals content. But, after bacterial treatment,
these values were reduced by 64.58 and 74.20%, respectively. It was observed that
40% untreated PMDE has no inhibitory effect on seed germination but 60, 80 and 100%
PMDE has inhibited 20, 40 and 60% germination, respectively while 100% germination
was recorded up to 60% treated PMDE. Moreover, 40 and 60% PMDE has shown deleterious
effects on seedling growth parameter and seeds treated with 80 and 100% PMDE showed
no root development. However, 20% bacteria treated PMDE was found most suitable for
plant growth possibly due to presence of optimum level of nutrients. Further, Phaseolus
seeds treated with 60 and 80% untreated PMDE showed reduced amylase activity and no
amylase activity was observed in seeds treated with 100% untreated PMDE. But, seeds
treated with bacterial degraded PMDE showed amylase activity and molecular weight
of alpha-amylase enzyme determined by SDS-PAGE was approximately 47.5, 46 and 44.5
kDa, respectively.