The Linearbandkeramik (LBK) settlement of Karsdorf (Saxony-Anhalt, Germany) revealed twenty-four longhouses and thirty-four associated burials. They were investigated in an interdisciplinary study focusing primarily on biological relationships and mobility within the community. Males, females, and subadults were buried individually or in groups in pits accompanying longhouses suggesting family relationships. The mitochondrial DNA (mtDNA), however, revealed only few biological relations among them. The rare potential relatives were found in different houses, indicating very low continuity of maternal lineages. Strontium isotope ratios of human tooth enamel point to differentiated land-use patterns or interaction of the Karsdorf community in the Unstrut river valley with people from settlements in typical loess locations. Representatives of both isotope ranges distinguished occur among all burial groups. The integrated interpretation of all data suggests exchange of people within consolidated networks of LBK neighbouring communities.