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      EAG response and behavioral orientation of Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) to synthetic host-associated volatiles

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          Abstract

          Dastarcus helophoroides Fairmaire (Coleoptera: Bothrideridae) is an effective predatory beetle of larvae and pupae of several cerambycid beetles including Monochamus alternatus and Anoplophora glabripennis. Electroantennography (EAG) and a dynamic two-choice olfactometer were respectively used to measure the antennal and behavioral responses of both sexes to selected volatile compounds. Female and male D. helophoroides exhibited similar EAG and behavioral responses. Significant dose-dependent EAG responses in both sexes were elicited by nonanal, octanal, cis-3-hexenol, 3-carene, (R)-(+)-α-pinene, (S)-(-)-α-pinene, (R)-(+)-limonene and (S)-(-)-limonene. Female and male beetles were repelled at high concentration by cis-3-hexenol and (S)-(-)-limonene, respectively. Both sexes of D. helophoroides were significantly attracted to nonanal, cis-3-hexenol, 3-carene and (R)-(+)-limonene even at low concentrations. These compounds might be used either individually or in mixtures for developing biological control methods to attract this predatory beetle into forest stands threatened by cerambycid beetles.

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          Ecology and management of exotic and endemic Asian longhorned beetleAnoplophora glabripennis

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            Chemo-electrical transduction in insect olfactory receptors.

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              Analysis of chemosensory gene families in the beetle Monochamus alternatus and its parasitoid Dastarcus helophoroides.

              We assembled antennal transcriptomes of pest Monochamus alternatus and its parasitoid Dastarcus helophoroides to identify the members of the major chemosensory multi-gene families. Gene ontology (GO) annotation indicated that the relative abundance of transcripts associated with specific GO terms was highly similar in the two species. In chemosensory gene families, we identified 52 transcripts encoding putative odorant-binding proteins (OBPs), 19 chemosensory proteins (CSPs), 10 olfactory receptors (ORs), 8 ionotropic receptors (IRs), 2 gustatory receptors (GRs), and 5 sensory neuron membrane proteins (SNMPs) in these two transcriptomes. Predicted protein sequences were compared with Dendroctonus ponderosae, Tribolium castaneum and Drosophila melanogaster. The results of phylogenetic trees showed that some clusters included only OBPs or CSPs from D. helophoroides, some clusters included only OBPs or CSPs from M. alternatus, while some clusters included OBPs or CSPs from both M. alternatus and D. helophoroides. The identification of the chemosensory genes and the phylogenetic relationship of these genes between two species might provide new ideas for controlling M. alternatus and improving current strategies for biological control.
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                Author and article information

                Contributors
                Role: Data curationRole: Formal analysisRole: InvestigationRole: MethodologyRole: SoftwareRole: Writing – original draft
                Role: MethodologyRole: ResourcesRole: Writing – review & editing
                Role: ConceptualizationRole: Funding acquisitionRole: ResourcesRole: SupervisionRole: Writing – review & editing
                Role: ConceptualizationRole: Funding acquisitionRole: ResourcesRole: SupervisionRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                21 December 2017
                2017
                : 12
                : 12
                : e0190067
                Affiliations
                [1 ] Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, P. R. China
                [2 ] Department of Forest Zoology and Forest Conservation, Georg-August-Universitat Gottingen, Göttingen, Germany
                USDA Agricultural Research Service, UNITED STATES
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                http://orcid.org/0000-0001-5306-8306
                Article
                PONE-D-17-14554
                10.1371/journal.pone.0190067
                5739483
                29267391
                a2a9fb3a-a3ab-4b0b-b9e9-75f0dcadc75d
                © 2017 Ren et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 14 April 2017
                : 7 December 2017
                Page count
                Figures: 4, Tables: 8, Pages: 18
                Funding
                Funded by: Forest Scientific Research in the Public Welfare
                Award ID: 201404401
                Award Recipient :
                Funded by: scientist exchange in the framework of the cooperation program PPP
                Award ID: 2009-6037
                Award Recipient :
                Funded by: BMBF-BEST project
                Award ID: FKZ033L033A-E
                Award Recipient :
                Funded by: Chinese National Natural Science Foundation
                Award ID: 31500529
                Award Recipient :
                This work was supported by Special Fund for Forest Scientific Research in the Public Welfare (201404401). We were also supported by the scientist exchange in the framework of the cooperation program PPP that was jointly funded by the CSC and DAAD (2009-6037), Chinese National Natural Science Foundation (31500529), and the BMBF-BEST project (FKZ033L033A-E). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
                Categories
                Research Article
                Biology and Life Sciences
                Organisms
                Eukaryota
                Animals
                Invertebrates
                Arthropoda
                Insects
                Beetles
                Physical Sciences
                Chemistry
                Chemical Compounds
                Silicones
                Biology and Life Sciences
                Biochemistry
                Lipids
                Oils
                Biology and Life Sciences
                Ecology
                Community Ecology
                Trophic Interactions
                Predation
                Ecology and Environmental Sciences
                Ecology
                Community Ecology
                Trophic Interactions
                Predation
                Biology and Life Sciences
                Behavior
                Animal Behavior
                Biology and Life Sciences
                Zoology
                Animal Behavior
                Biology and Life Sciences
                Zoology
                Animal Anatomy
                Antennae (Animal Physiology)
                Biology and Life Sciences
                Zoology
                Animal Physiology
                Antennae (Animal Physiology)
                Biology and Life Sciences
                Ecology
                Ecosystems
                Forests
                Ecology and Environmental Sciences
                Ecology
                Ecosystems
                Forests
                Ecology and Environmental Sciences
                Terrestrial Environments
                Forests
                Biology and Life Sciences
                Organisms
                Eukaryota
                Plants
                Trees
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                All relevant data are within the paper.

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