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      Draft Genome Sequence of Clostridium bifermentans Strain WYM, a Promising Biohydrogen Producer Isolated from Landfill Leachate Sludge

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          Abstract

          Clostridium bifermentans strain WYM is an effective biohydrogen producer isolated from landfill leachate sludge. Here, we present the assembly and annotation of its genome, which may provide further insights into the metabolic pathways involved in efficient biohydrogen production.

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          Most cited references11

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          tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence

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            Producing hydrogen from wastewater sludge by Clostridium bifermentans.

            Excess wastewater sludge collected from the recycling stream of an activated sludge process is biomass that contains large quantities of polysaccharides and proteins. However, relevant literature indicates that the bio-conversion of wastewater sludge to hydrogen is limited and therefore not economically feasible. This work examined the anaerobic digestion of wastewater sludge using a clostridium strain isolated from the sludge as inoculum. A much higher hydrogen yield than presented in the literature was obtained. Also, the effects of five pre-treatments-ultrasonication, acidification, sterilization, freezing/thawing and adding methanogenic inhibitor-on the production of hydrogen were examined. Freezing and thawing and sterilization increased the specific hydrogen yield by 1.5-2.5 times to that of untreated sludge, while adding an inhibitor and ultrasonication reduced the hydrogen yield.
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              Clostridium bifermentans bacteremia with metastatic osteomyelitis.

              Osteomyelitis caused solely by an anaerobic organism is uncommon. We report a case of recurrent Clostridium bifermentans bacteremia resulting in metastatic osteomyelitis involving the sacrum, spine, and ribs. The emergence of resistance of this organism to imipenem and metronidazole is noteworthy because of the usual susceptibility of clostridial species to these antibiotics.
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                Author and article information

                Journal
                Genome Announc
                Genome Announc
                ga
                ga
                GA
                Genome Announcements
                American Society for Microbiology (1752 N St., N.W., Washington, DC )
                2169-8287
                6 March 2014
                Mar-Apr 2014
                : 2
                : 2
                : e00077-14
                Affiliations
                [a ]School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Malaysia
                [b ]Nanotechnology & Catalysis Research Centre (NANOCAT), University of Malaya, Kuala Lumpur, Malaysia
                Author notes
                Address correspondence to J. C. Juan, jcjuan@ 123456um.edu.my .
                Article
                genomeA00077-14
                10.1128/genomeA.00077-14
                3945495
                24604639
                8e111aca-9f9c-4dbd-a5ed-b7c48ac3c06a
                Copyright © 2014 Wong et al.

                This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

                History
                : 24 January 2014
                : 28 January 2014
                Page count
                Pages: 2
                Categories
                Prokaryotes
                Custom metadata
                March/April 2014
                free

                Genetics
                Genetics

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