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Abstract
The hyper-activation of PI3K/AKT signaling is common in many kinds of malignant tumors
and promotes cell growth. Moreover, FBXL5 is reported to play an important role in
the progression of gastric cancer and cervical cancer. In this view, this study aims
to explore the function of FBXL5 in the progression of colon cancer and determine
if PI3K/AKT signaling pathway involves in this process. Western blotting, RT-PCR,
and immunohistochemistry were used to detect the expression pattern of FBXL5 in colon
cancer tissues and cell lines. Immunofluorescence, Duolink, and immunoprecipitation
(IP) assays were performed to evaluate the interaction between FBXL5 and PI3K/AKT
signaling. Results showed that FBXL5 was elevated in colon cancer tissues and cells,
which had physical interaction with PTEN protein and negatively regulated its expression,
whereas positively modulated PI3K, AKT and mTOR expression and their phosphorylation.
Besides, FBXL5 promoted cell proliferation and tumorigenesis and inhibited apoptosis
by modulating PTEN/PI3K/AKT signaling. In conclusion, this study demonstrated that
FBXL5 functioned as an oncogene in the progression of colon cancer through regulating
PTEN/PI3K/AKT signaling.