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      Alginate Materials and Dental Impression Technique: A Current State of the Art and Application to Dental Practice

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          Abstract

          Hydrocolloids were the first elastic materials to be used in the dental field. Elastic impression materials include reversible (agar-agar), irreversible (alginate) hydrocolloids and synthetic elastomers (polysulfides, polyethers, silicones). They reproduce an imprint faithfully, providing details of a high definition despite the presence of undercuts. With the removal of the impression, being particularly rich in water, the imprints can deform but later adapt to the original shape due to the elastic properties they possess. The advantages of using alginate include the low cost, a better tolerability on the part of the patient, the ease of manipulation, the short time needed for execution, the instrumentation and the very simple execution technique and possibility of detecting a detailed impression (even in the presence of undercuts) in a single step. A comprehensive review of the current literature was conducted according to the PRISMA guidelines by accessing the NCBI PubMed database. Authors conducted a search of articles in written in English published from 2008 to 2018. All the relevant studies were included in the search with respect to the characteristics and evolution of new marine derived materials. Much progress has been made in the search for new marine derived materials. Conventional impression materials are different, and especially with the advent of digital technology, they have been suffering from a decline in research attention over the last few years. However, this type of impression material, alginates (derived from marine algae), have the advantage of being among the most used in the dental medical field.

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          Most cited references50

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          Engineering vascularized skeletal muscle tissue.

          One of the major obstacles in engineering thick, complex tissues such as muscle is the need to vascularize the tissue in vitro. Vascularization in vitro could maintain cell viability during tissue growth, induce structural organization and promote vascularization upon implantation. Here we describe the induction of endothelial vessel networks in engineered skeletal muscle tissue constructs using a three-dimensional multiculture system consisting of myoblasts, embryonic fibroblasts and endothelial cells coseeded on highly porous, biodegradable polymer scaffolds. Analysis of the conditions for induction and stabilization of the vessels in vitro showed that addition of embryonic fibroblasts increased the levels of vascular endothelial growth factor expression in the construct and promoted formation and stabilization of the endothelial vessels. We studied the survival and vascularization of the engineered muscle implants in vivo in three different models. Prevascularization improved the vascularization, blood perfusion and survival of the muscle tissue constructs after transplantation.
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            3D Cell Culture in Alginate Hydrogels

            This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choice of gelation technique (ionic or covalent), and divalent cation chosen as the gel inducing ion. The use of peptide-coupled alginate can control cell–matrix interactions. Gelation of alginate with concomitant immobilization of cells can take various forms. Droplets or beads have been utilized since the 1980s for immobilizing cells. Newer matrices such as macroporous scaffolds are now entering the 3D cell culture product market. Finally, delayed gelling, injectable, alginate systems show utility in the translation of in vitro cell culture to in vivo tissue engineering applications. Alginate has a history and a future in 3D cell culture. Historically, cells were encapsulated in alginate droplets cross-linked with calcium for the development of artificial organs. Now, several commercial products based on alginate are being used as 3D cell culture systems that also demonstrate the possibility of replacing or regenerating tissue.
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              Intraoral Digital Impression Technique: A Review.

              With the techniques of computer-aided design and computer-aided manufacturing (CAD/CAM) being applied in the field of prosthodontics, a concept of intraoral digital impressions was put forward in the early 1980s. It has drawn comprehensive attention from dentists and has been used for dental prosthesis fabrication in a number of cases. This new digital impression technique is expected to bring about absolute digitization to the mode of prosthodontics. A few published articles have indicated that dental prostheses fabricated from intraoral digital impressions have exhibited remarkable advantages over those from conventional impressions in several respects. The present review discusses intraoral digital impression techniques in terms of the following aspects: (1) categories and principles of intraoral digital impression devices currently available; (2) operating characteristics of the devices; and (3) comparison of the manipulation, accuracy, and repeatability between intraoral digital impression and conventional impression.
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                Author and article information

                Journal
                Mar Drugs
                Mar Drugs
                marinedrugs
                Marine Drugs
                MDPI
                1660-3397
                29 December 2018
                January 2019
                : 17
                : 1
                : 18
                Affiliations
                [1 ]Department of Biomedical and Dental Sciences and Morphological and Functional Imaging, Messina University, 98100 ME Messina, Italy; gcervino@ 123456unime.it (G.C.); lucafiorillo@ 123456live.it (L.F.); amoroso.giulia@ 123456hotmail.com (G.A.); matamarco94@ 123456gmail.com (M.M.); cesaredamico89@ 123456gmail.com (C.D.); enastrosiniscalchi@ 123456unime.it (E.N.S.)
                [2 ]Department of Maxillofacial Surgery, Loma Linda University, Loma Linda, CA 92354, USA; aherford@ 123456llu.edu
                [3 ]Multidisciplinary Department of Medical-Surgical and Odontostomatological Specialties, University of Campania “Luigi Vanvitelli”, 80100 Naples, Italy; luigi.laino@ 123456unicampania.it
                [4 ]Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy; giuseppe.troiano@ 123456unifg.it
                [5 ]Department of Surgical and Biomedical Sciences, Catania University, 95123 Catania, Italy; torecrimi@ 123456gmail.com
                Author notes
                [* ]Correspondence: mcicciu@ 123456unime.it or acromarco@ 123456yahoo.it ; Tel.: +39-0902216920; Fax: +39-0902216921
                Author information
                https://orcid.org/0000-0003-4619-4691
                https://orcid.org/0000-0003-0335-4165
                https://orcid.org/0000-0001-5647-4414
                https://orcid.org/0000-0003-2311-9728
                Article
                marinedrugs-17-00018
                10.3390/md17010018
                6356954
                30597945
                7bb2ac56-e245-434a-92d1-404208ce2bdc
                © 2018 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                : 25 November 2018
                : 24 December 2018
                Categories
                Review

                Pharmacology & Pharmaceutical medicine
                impression materials,marine derivates,alginates,marine algae

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