Distribution of bla OXA-23, ISAba, Aminoglycosides resistant genes among burned & ICU patients in Tehran and Sari, Iran

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

Multidrug resistant strains of Acinetobacter baumannii (MDR-AB) have emerged as alarming nosocomial pathogens among patients admitted to Intensive Care Unit and burned patients. The aim of this study was to determine the susceptibility of A. baumannii isolates, the carbapenems resistance patterns bla ^OXA-23 and also IS _Aba elements of A. baumannii isolates among burned and ICU patients in Tehran and Sari, Iran.

Methods

In this study, 100 A. baumannii isolates from burned and ICU patients in Tehran and Sari (Iran) during 2013 were tested for determination of antimicrobials susceptibility by the disc-diffusion method on Mueller Hinton agar recommended by the guidelines of Clinical and Laboratory Standards Institute (CLSI), and frequency bla ^OXA-23 carbapenemase genes, and insertion elements IS _Aba genes were studied by PCR method.

Results

The highest rates of susceptibility were observed with Colistin (88.7%), Tigecycline (82.2%), Imipenem (67%) and IS _Aba (32.2%). The extensively drug-resistance and pan drug-resistance were observed in 37.1% and 8.1% isolates, respectively.

Results indicated among isolates resistant to Aminoglycoside and Carbapenem, the highest resistance was observed to Streptomycin (90%) ‚ and the most sensitivity was to Imipenem (67%).

Conclusions

This is the most study that attempted to detect Acinetobacter baumanii the insertion elements IS _Aba, bla ^OXA-23 and aminoglycosides resistance in MDR-AB isolates from burned and ICU patients in Iran. In a timely manner, antimicrobial resistance surveillance and strict infection control strategies are still lacking in burn ward and ICU in Iran, despite the alarming emergence of MDR-AB strains, particularly among those isolates that are not susceptible to Colistin. The results of this study are consistent with a recent report in which a number of combinations exhibited potent activity against Multidrug resistant strains of A. baumannii (MDR-AB).

Related collections

Most cited references 15

Record: found
Abstract: found
Article: not found

Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options.

Lisa Maragakis, Trish Perl (2008)

Multidrug-resistant Acinetobacter baumannii is recognized to be among the most difficult antimicrobial-resistant gram-negative bacilli to control and treat. Increasing antimicrobial resistance among Acinetobacter isolates has been documented, although definitions of multidrug resistance vary in the literature. A. baumannii survives for prolonged periods under a wide range of environmental conditions. The organism causes outbreaks of infection and health care-associated infections, including bacteremia, pneumonia, meningitis, urinary tract infection, and wound infection. Antimicrobial resistance greatly limits the therapeutic options for patients who are infected with this organism, especially if isolates are resistant to the carbapenem class of antimicrobial agents. Because therapeutic options are limited for multidrug-resistant Acinetobacter infection, the development or discovery of new therapies, well-controlled clinical trials of existing antimicrobial regimens and combinations, and greater emphasis on the prevention of health care-associated transmission of multidrug-resistant Acinetobacter infection are essential.

0 comments Cited 301 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Global spread of carbapenem-resistant Acinetobacter baumannii.

Paul Higgins, Cathrin Dammhayn, Meredith Hackel … (2010)

We have investigated the molecular epidemiology and distribution of carbapenemase genes in 492 imipenem-non-susceptible Acinetobacter baumannii worldwide isolates (North and Latin America, Europe, Asia, South Africa and Australia). MICs were determined by broth microdilution and Etest. The presence of carbapenemase-encoding genes was investigated by PCR. Molecular epidemiology was performed by repetitive sequence-based PCR (rep-PCR; DiversiLab), sequence-type multiplex PCR and PFGE. Imipenem non-susceptibility was associated with ISAba1 upstream of the intrinsic bla(OXA-51-like) or the acquired carbapenemase bla(OXA-23-like), bla(OXA-40-like) or bla(OXA-58-like). Isolates were grouped into eight distinct clusters including European clones I, II and III. European clone II was the largest (246 isolates) and most widespread group (USA, pan-Europe, Israel, Asia, Australia and South Africa). The global dissemination of eight carbapenem-resistant lineages illustrates the success this organism has had in epidemic spread. The acquired OXA enzymes are widely distributed but are not the sole carbapenem resistance determinant in A. baumannii.

0 comments Cited 187 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii.

Jane F Turton, M Ward, Neil Woodford … (2006)

ISAba1 was found in all widespread clones of Acinetobacter baumannii in the United Kingdom. All isolates studied had a blaOXA-51-like carbapenemase gene; some also had blaOXA-23-like and/or blaOXA-58-like. Among isolates with blaOXA-51-like as sole carbapenemase gene, only those with ISAba1 adjacent to blaOXA-51-like were carbapenem resistant. Minor differences in blaOXA-51-like sequence were observed in resistant and susceptible isolates. Isolates with blaOXA-23-like in addition were consistently resistant to carbapenems; in all of these ISAba1 lay upstream of blaOXA-23-like, but was not associated with blaOXA-51-like. These results suggest that ISAba1 is providing the promoter for blaOXA-51-like and, probably, for blaOXA-23-like.

0 comments Cited 174 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Mohtaram Nasrolahei: mnasrolahei@yahoo.com

Bahador Zahedi: zahedi.bahador@gmail.com

Abbas Bahador: ab.bahador@gmail.com

Hossein Saghi: saghi1443@gmail.com

Soudeh Kholdi: soudehkholdi@yahoo.com

Neda Jalalvand: nedajalalvand@gmail.com

Davoud Esmaeili: esm114@gmail.com

Journal

Journal ID (nlm-ta): Ann Clin Microbiol Antimicrob

Journal ID (iso-abbrev): Ann. Clin. Microbiol. Antimicrob

Title: Annals of Clinical Microbiology and Antimicrobials

Publisher: BioMed Central (London )

ISSN (Electronic): 1476-0711

Publication date (Electronic): 25 September 2014

Publication date Collection: 2014

Volume: 13

Electronic Location Identifier: 38

Affiliations

[ ]Department of Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

[ ]Departments of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

[ ]Applied Microbiology Research center and Microbiology Department, Baqiyatallah University Medical of Sciences, Tehran, Iran

[ ]Department of Genetic‚ Science and Research Branch, Islamic Azad University, Tabriz, East Azerbaijan Iran

Article

Publisher ID: 38

DOI: 10.1186/s12941-014-0038-0

PMC ID: 4353670

PubMed ID: 25252850

SO-VID: 79dab63e-3778-4c79-a652-0c61ac43ed9b

License:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 21 January 2014

Date accepted : 16 July 2014

Custom metadata

ScienceOpen disciplines: Infectious disease & Microbiology

Keywords: acinetobacter baumannii‚ bla oxa-23 ‚ isaba,carbapenemase,aminoglycoside

Data availability:

ScienceOpen disciplines: Infectious disease & Microbiology

Keywords: acinetobacter baumannii‚ bla oxa-23 ‚ isaba, carbapenemase, aminoglycoside