Immunogenicity and protective efficacy of Salmonella enterica serovar Pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Background

Salmonella enterica serovar Pullorum ( S. Pullorum) causes Pullorum disease (PD), a severe systemic disease of poultry and results in considerable economic losses in developing countries. In order to develop a safe and immunogenic vaccine, the immunogenicity and protective efficacy of S06004ΔSPI2, a Salmonella pathogenicity island 2 (SPI2) deleted mutant of S. Pullorum was evaluated in 2-day old chickens.

Results

Single intramuscular vaccination with S06004ΔSPI2 (2 × 10 ⁷ CFU) of chickens revealed no differences in body weight or clinical symptoms compared to control group. S06004ΔSPI2 bacteria can colonize and persistent in liver and spleen of vaccinated chickens approximately 14 days, and specific humoral and cellular immune responses were significantly induced. Vaccination of chickens offered efficient protection against S. Pullorum strain S06004 and S. Gallinarum strain SG9 challenge, respectively, at 10 days post vaccination (dpv) based on mortality and clinical symptoms compared to control group.

Conclusions

These findings suggest that S06004ΔSPI2 appears to be a highly immunogenic and efficient live attenuated vaccine candidate.

Related collections

Most cited references 25

Record: found
Abstract: found
Article: not found

Salmonella interactions with host cells: type III secretion at work.

J Galán (2000)

The bacterial pathogen Salmonella enterica has evolved a very sophisticated functional interface with its vertebrate hosts. At the center of this interface is a specialized organelle, the type III secretion system, that directs the translocation of bacterial proteins into the host cell. Salmonella spp. encode two such systems that deliver a remarkable array of bacterial proteins capable of modulating a variety of cellular functions, including actin cytoskeleton dynamics, nuclear responses, and endocytic trafficking. Many of these bacterial proteins operate by faithful mimicry of host proteins, in some cases representing the result of extensive molecular tinkering and convergent evolution. The coordinated action of these type III secreted proteins secures the replication and survival of the bacteria avoiding overt damage to the host. The study of this remarkable pathogen is not only illuminating general paradigms in microbial pathogenesis but is also providing valuable insight into host cell functions.

0 comments Cited 157 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival.

D M Cirillo, R. Valdivia, D M Monack … (1998)

Salmonella pathogenicity island 2 (SPI-2) encodes a putative type III secretion system necessary for systemic infection in animals. We have investigated the transcriptional organization and regulation of SPI-2 by creating gfp fusions throughout the entire gene cluster. These gfp fusions demonstrated that SPI-2 genes encoding structural, regulatory and previously uncharacterized putative secreted proteins are preferentially expressed in the intracellular environment of the host macrophage. Furthermore, the transcription of these genes within host cells was dependent on the two-component regulatory system SsrA/SsrB and an acidic phagosomal environment. Most SPI-2 mutants failed to replicate to the same level as wild-type strains in murine macrophages and human epithelial cells. In orally infected mice, SPI-2 mutants colonized the Peyer's patches but did not progress to the mesenteric lymph nodes. We conclude that SPI-2 genes are specifically expressed upon entry into mammalian cells and are required for intracellular growth in host cells in vivo and in vitro.

0 comments Cited 148 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Pullorum disease and fowl typhoid--new thoughts on old diseases: a review.

P Barrow, O Freitas Neto (2011)

Fowl typhoid and pullorum disease are two distinct septicaemic diseases largely specific to avian species and caused by Salmonella Gallinarum and Salmonella Pullorum, respectively. They were first described more than one century ago. Since their discovery, many efforts have been made to control and prevent their occurrence in commercial farming of birds. However, they remain a serious economic problem to livestock in countries where measures of control are not efficient or in those where the climatic conditions favour the environmental spread of these microorganisms. During the past 15 to 20 years there has been an explosion of genetic and immunological information on the biology of these two organisms, which is beginning to contribute to a better understanding of the organisms and their interaction with the host. However, it is not enough simply to understand the pathology in greater and greater detail. What is needed, in addition to this increase in basic knowledge, is creative thinking to challenge existing paradigms and to develop really novel approaches to infection control.

0 comments Cited 95 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Xinan Jiao: +86-514-87971803 , jiao@yzu.edu.cn

Journal

Journal ID (nlm-ta): BMC Vet Res

Journal ID (iso-abbrev): BMC Vet. Res

Title: BMC Veterinary Research

Publisher: BioMed Central (London )

ISSN (Electronic): 1746-6148

Publication date (Electronic): 24 July 2015

Publication date PMC-release: 24 July 2015

Publication date Collection: 2015

Volume: 11

Electronic Location Identifier: 162

Affiliations

Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu 225009 P R China

Article

Publisher ID: 497

DOI: 10.1186/s12917-015-0497-3

PMC ID: 4513372

SO-VID: 70748f76-2359-4e62-b8a7-9c6f35384ef8

License:

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 30 March 2015

Date accepted : 16 July 2015

Custom metadata

ScienceOpen disciplines: Veterinary medicine

Keywords: salmonella enterica serovar pullorum,pullorum disease,salmonella pathogenicity island 2,live attenuated vaccine

Data availability:

ScienceOpen disciplines: Veterinary medicine

Keywords: salmonella enterica serovar pullorum, pullorum disease, salmonella pathogenicity island 2, live attenuated vaccine

Immunogenicity and protective efficacy of Salmonella enterica serovar Pullorum pathogenicity island 2 mutant as a live attenuated vaccine candidate

Read this article at

Abstract

Background

Results

Conclusions

Related collections

Horses and Humans Research Foundation

Most cited references 25

Salmonella interactions with host cells: type III secretion at work.

Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival.

Pullorum disease and fowl typhoid--new thoughts on old diseases: a review.

Author and article information

Contributors

Journal

Affiliations

Article

History

Categories

Custom metadata

Comments

Comment on this article

Similar content 164

Cited by 2

Most referenced authors 704