Isoleucilactucin Ameliorates Coal Fly Ash-Induced Inflammation through the NF-κB and MAPK Pathways in MH-S Cells

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Abstract

We investigated whether isoleucilactucin, an active constituent of Ixeridium dentatum, reduces inflammation caused by coal fly ash (CFA) in alveolar macrophages (MH-S). The anti-inflammatory effects of isoleucilactucin were assessed by measuring the concentration of nitric oxide (NO) and the expression of pro-inflammatory mediators in MH-S cells exposed to CFA-induced inflammation. We found that isoleucilactucin reduced CFA-induced NO generation dose-dependently in MH-S cells. Moreover, isoleucilactucin suppressed CFA-activated proinflammatory mediators, including cyclooxygenase-2 (COX2) and inducible NO synthase (iNOS), and the proinflammatory cytokines such as interleukin-(IL)-1β, IL-6, and tumor necrosis factor (TNF-α). The inhibiting properties of isoleucilactucin on the nuclear translocation of phosphorylated nuclear factor-kappa B (p-NF-κB) were observed. The effects of isoleucilactucin on the NF-κB and mitogen-activated protein kinase (MAPK) pathways were also measured in CFA-stimulated MH-S cells. These results indicate that isoleucilactucin suppressed CFA-stimulated inflammation in MH-S cells by inhibiting the NF-κB and MAPK pathways, which suggest it might exert anti-inflammatory properties in the lung.

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Author and article information

Contributors

H. M. Arif Ullah: (View ORCID Profile)

SeonJu Park: (View ORCID Profile)

Sung Dae Kim: (View ORCID Profile)

Man Hee Rhee: (View ORCID Profile)

Journal

Journal ID (publisher-id): IJMCFK

Title: International Journal of Molecular Sciences

Abbreviated Title: IJMS

Publisher: MDPI AG

ISSN (Electronic): 1422-0067

Publication date Created: September 2021

Publication date (Electronic): September 01 2021

Volume: 22

Issue: 17

Page: 9506

Article

DOI: 10.3390/ijms22179506

SO-VID: 5dc705f2-f682-4ede-bfd8-890efd506023

License:

https://creativecommons.org/licenses/by/4.0/

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