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      Two-thirds of pregnant mothers attending antenatal care services at Arsi Zone, Oromia Regional State, Ethiopia had no comprehensive knowledge of HIV/AIDS: A cross-sectional study

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          Abstract

          Objective:

          The study aimed to assess the magnitude and factors associated with comprehensive knowledge of HIV/AIDS among pregnant mothers attending antenatal care (ANC) services at health institutions in Arsi zone, Oromia Regional State, Ethiopia.

          Methods:

          The study employed a health facility-based cross-sectional study design from 18 March to 25 June 2019. Out of an estimated sample size of 4481, a total of 4440 (92.23%) pregnant women were selected by multistage random sampling technique and interviewed. Multivariable binary logistic regression was used to identify the factors associated with comprehensive knowledge of HIV/AIDS. The model fitness was tested by Hosmer and Lemeshow goodness of fit, which provided a p value of 0.72 and deviance reduced (i.e., −2log likelihood was reduced from 5580.38 to 5069.55 with a p value of 0.000).

          Results:

          Out of the total mothers (4440) interviewed, only 1430 (32.2%; 95% confidence interval: (30.83%, 33.60%)) had comprehensive knowledge about HIV/AIDS. Not knowing safe period to be pregnant (adjusted odds ratio = 0.67; 95% confidence interval: 0.56, 0.81), and not empowering women’s for sexual practice (adjusted odds ratio = 0.50; 95% confidence interval: 0.43, 0.58) let women have less comprehensive knowledge while women who never educated about the sexual matter (adjusted odds ratio = 1.65; 95% confidence interval: 1.42, 1.92), who had a discouraging attitude toward having multiple partners (adjusted odds ratio = 1.53; 95% confidence interval: 1.24, 1.88), who had a discouraging attitude toward premarital sex (adjusted odds ratio = 1.68; 95% confidence interval: 1.38, 2.03), and who had a positive attitude toward accepting HIV/AIDS patients (2.69; 95% confidence interval: 2.34, 3.10) had more comprehensive knowledge of HIV/AIDS.

          Conclusion:

          Only about one-third (32.2%) of pregnant mothers on ANC follow-up had comprehensive knowledge. Thus, it would be better if the health institutions emphasize educating the mothers attending antenatal care follow-up about HIV/AIDS, for those who do not know pregnancy occurrence date, not empowered of sexual practice, and had an encouraging attitude toward multiple partners, premarital sex, and negative attitude toward accepting HIV/AIDS patients.

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          Most cited references25

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          Opening the archives for state of the art tumour genetic research: sample processing for array-CGH using decalcified, formalin-fixed, paraffin-embedded tissue-derived DNA samples

          Background Molecular genetic studies on rare tumour entities, such as bone tumours, often require the use of decalcified, formalin-fixed, paraffin-embedded tissue (dFFPE) samples. Regardless of which decalcification procedure is used, this introduces a vast breakdown of DNA that precludes the possibility of further molecular genetic testing. We set out to establish a robust protocol that would overcome these intrinsic hurdles for bone tumour research. Findings The goal of our study was to establish a protocol, using a modified DNA isolation procedure and quality controls, to select decalcified samples suitable for array-CGH testing. Archival paraffin blocks were obtained from 9 different pathology departments throughout Europe, using different fixation, embedding and decalcification procedures, in order to preclude a bias for certain lab protocols. Isolated DNA samples were subjected to direct chemical labelling and enzymatic labelling systems and were hybridised on a high resolution oligonucleotide chip containing 44,000 reporter elements. Genomic alterations (gains and losses) were readily detected in most of the samples analysed. For example, both homozygous deletions of 0.6 Mb and high level of amplifications of 0.7 Mb were identified. Conclusions We established a robust protocol for molecular genetic testing of dFFPE derived DNA, irrespective of fixation, decalcification or sample type used. This approach may greatly facilitate further genetic testing on rare tumour entities where archival decalcified, formalin fixed samples are the only source.
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            Global action to reduce HIV stigma and discrimination

            There is no question that the stigma and discrimination associated with HIV and AIDS can be reduced through intervention. The inclusion of stigma and discrimination reduction as a critical component of achieving an AIDS-free generation in recent UNAIDS, UN and PEPFAR political initiatives is promising. Yet national governments need evidence on effective interventions at the individual, community and societal levels in order to strategically incorporate stigma and discrimination reduction into national AIDS plans. Currently, the heterogeneity of stigma and discrimination reduction approaches and measurement makes it challenging to compare and contrast evaluated interventions. Moving forward, it is critical for the research community to: (1) clearly link intervention activities to the domains of stigma to be shifted; (2) assess the stigma domains in a consistent manner; and (3) link stigma and discrimination reduction with HIV prevention, care and treatment outcomes (e.g., uptake, adherence and retention of ART). These steps would further advance the scientific evidence base of stigma and discrimination reduction and allow for the identification of effective interventions that could be scaled up by national governments.
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              Multiple shRNA combinations for near-complete coverage of all HIV-1 strains

              Background Combinatorial RNA interference (co-RNAi) approaches are needed to account for viral variability in treating HIV-1 with RNAi, as single short hairpin RNAs (shRNA) are rapidly rendered ineffective by resistant strains. Current work suggests that 4 simultaneously expressed shRNAs may prevent the emergence of resistant strains. Results In this study we assembled combinations of highly-conserved shRNAs to target as many HIV-1 strains as possible. We analyzed intersecting conservations of 10 shRNAs to find combinations with 4+ matching the maximum number of strains using 1220+ HIV-1 sequences from the Los Alamos National Laboratory (LANL). We built 26 combinations of 2 to 7 shRNAs with up to 87% coverage for all known strains and 100% coverage of clade B subtypes, and characterized their intrinsic suppressive activities in transient expression assays. We found that all combinations had high combined suppressive activities, though there were also large changes in the individual activities of the component shRNAs in our multiple expression cassette configurations. Conclusion By considering the intersecting conservations of shRNA combinations we have shown that it is possible to assemble combinations of 6 and 7 highly active, highly conserved shRNAs such that there is always at least 4 shRNAs within each combination covering all currently known variants of entire HIV-1 subtypes. By extension, it may be possible to combine several combinations for complete global coverage of HIV-1 variants.
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                Author and article information

                Journal
                SAGE Open Med
                SAGE Open Med
                SMO
                spsmo
                SAGE Open Medicine
                SAGE Publications (Sage UK: London, England )
                2050-3121
                20 January 2023
                2023
                : 11
                : 20503121221145614
                Affiliations
                [1 ]Department of Public Health, College of Health Sciences, Arsi University, Asella Town, Oromia Regional state, Ethiopia
                [2 ]Department of Clinical Nursing, College of Health Sciences, Arsi University, Asella Town, Oromia Regional State, Ethiopia
                Author notes
                [*]Abenet Menene Gurara, Department of Clinical Nursing, College of Health Science, Arsi University, P.O.Box 193/04, Oromia Regional State, Asella, Ethiopia. Email: abenetmen@ 123456gmail.com
                Author information
                https://orcid.org/0000-0002-3973-1455
                Article
                10.1177_20503121221145614
                10.1177/20503121221145614
                9893063
                4c69337b-ba93-4a92-8ae2-33c00f7b58de
                © The Author(s) 2023

                This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages ( https://us.sagepub.com/en-us/nam/open-access-at-sage).

                History
                : 10 September 2021
                : 29 November 2022
                Funding
                Funded by: Arsi university, College of Health Sciences, ;
                Award ID: A/CHS/R001/15/16
                Categories
                Original Article
                Custom metadata
                January-December 2023
                ts1

                comprehensive knowledge of hiv/aids,mothers’ hiv/aids knowledge,mothers attending anc

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