Growth, ammonium metabolism, and photosynthetic properties of Ulva australis (Chlorophyta) under decreasing pH and ammonium enrichment

Abstract

The responses of macroalgae to ocean acidification could be altered by availability of macronutrients, such as ammonium (NH ₄ ⁺). This study determined how the opportunistic macroalga, Ulva australis responded to simultaneous changes in decreasing pH and NH ₄ ⁺ enrichment. This was investigated in a week-long growth experiment across a range of predicted future pHs with ambient and enriched NH ₄ ⁺ treatments followed by measurements of relative growth rates (RGR), NH ₄ ⁺ uptake rates and pools, total chlorophyll, and tissue carbon and nitrogen content. Rapid light curves (RLCs) were used to measure the maximum relative electron transport rate (rETR _max) and maximum quantum yield of photosystem II (PSII) photochemistry (F _v/F _m). Photosynthetic capacity was derived from the RLCs and included the efficiency of light harvesting (α), slope of photoinhibition (β), and the light saturation point (E _k). The results showed that NH ₄ ⁺ enrichment did not modify the effects of pH on RGRs, NH ₄ ⁺ uptake rates and pools, total chlorophyll, rETR _max, α, β, F _v/F _m, tissue C and N, and the C:N ratio. However, E _k was differentially affected by pH under different NH ₄ ⁺ treatments. E _k increased with decreasing pH in the ambient NH ₄ ⁺ treatment, but not in the enriched NH ₄ ⁺ treatment. NH ₄ ⁺ enrichment increased RGRs, NH ₄ ⁺ pools, total chlorophyll, rETR _max, α, β, F _v/F _m, and tissue N, and decreased NH ₄ ⁺ uptake rates and the C:N ratio. Decreased pH increased total chlorophyll content, rETR _max, F _v/F _m, and tissue N content, and decreased the C:N ratio. Therefore, the results indicate that U. australis growth is increased with NH ₄ ⁺ enrichment and not with decreasing pH. While decreasing pH influenced the carbon and nitrogen metabolisms of U. australis, it did not result in changes in growth.