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      "In vitro" activity of gentian violet against asexual Plasmodium falciparum parasites Translated title: Ação, "in vitro". Da violeta de genciana sobre formas evolutivas do plasmodium falciparum

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          Abstract

          In order to investigate whether gentian violet exhibited "in vitro" inhibitory activity against Plasmodium falciparum, the Authors have carried out 20 sensitivity tests according to the microtechnique described by RIECK MANN et al.5. Results have shown inhibition of schizonts'maturation at the following concentration: 1/1000; 1/1500; 1/2000; 1/2500; 1/3000 and 1/4000, thus demonstrating inhibitory activity of the tested dye against asexual blood parasites. The present data suggest gentain violet may be possibly used in the prophylaxis of transfusion-acquired malaria.

          Translated abstract

          Utilizando a técnica de RIECKMANN e col.5, os autores realizaram 20 microtestes de sensibilidade, procurando verificar a capacidade da violeta de genciana em impedir, na cultura "in vitro", o desenvolvimento habitual do Plasmodium falciparum. Os resultados mostraram que houve inibiçáo da evolução do protozoário nas concentrações de 1/1000, 1/1500, 1/2000, 1/2500, 1/3000 e 1/4000, significando que nas condições da experiência o corante atuou sobre as formas sangüíneas assexuadas do protozoário. Estas verificações sugerem que a violeta de genciana poderia ser usada na profilaxia da malária transfusional.

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          Human malaria parasites in continuous culture

          Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. The have remained infective to Aotus.
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            Drug sensitivity of plasmodium falciparum. An in-vitro microtechnique.

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              Transfusion malaria revisited.

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                Author and article information

                Journal
                rimtsp
                Revista do Instituto de Medicina Tropical de São Paulo
                Rev. Inst. Med. trop. S. Paulo
                Instituto de Medicina Tropical de São Paulo (São Paulo, SP, Brazil )
                1678-9946
                February 1988
                : 30
                : 1
                : 17-20
                Affiliations
                [03] orgnameUniversidade de São Paulo orgdiv1Faculdade de Medicina orgdiv2Departamento de Dozens Infecciosas e Parasitárias
                [02] São Paulo orgnameSecretaria de Estado da Saúde orgdiv1Superintendência do Controle de Endemias
                [01] orgnameLaboratório de Investigação Médica Parasitologia
                Article
                S0036-46651988000100003 S0036-4665(88)03000103
                10.1590/S0036-46651988000100003
                42ee4d27-011d-482f-809f-0835c61318fd

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 03 August 1987
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 7, Pages: 4
                Product

                SciELO Brazil

                Categories
                Original Articles

                Plasmodium falciparum,Malária transfusional-profilaxia,Violeta de genciana - ação "in vitro"

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