Two haplotypes of Capsella bursa-pastoris (Brassicaceae) in Continental Chile support multiple introduction  <span class="so-article-trans-title" dir="auto"> Translated title: Dos haplotipos de Capsella bursa-pastoris en Chile continental soportan múltiple introducción </span>

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Abstract

Shepherd’s purse (Capsella bursa-pastoris; Brassicaceae) is a global weed. In Chile, this species was introduced early during Spanish colonization, however, previous studies based on allozymes and RAPD fragments, show that populations in southernmost Chile could be related to England populations. Here, we analyze the geographical variation of three sequences of cpSSR in 286 individuals of C. bursa-pastoris (ATCP46615, ATCP66701 and ATCP31017). We collected plants from 14 populations distributed over a wide latitudinal gradient in continental Chile. Only one of the markers showed variation (ATCP31017), evidencing the presence of two haplotypes along the territory. The geographic distribution of these haplotypes support previous studies based on allozymes and RAPD fragments, suggesting that C. bursa-pastoris in continental Chile was introduced at least twice.

Translated abstract

La bolsita de pastor (Capsella bursa-pastoris, Brassicaceae) es una maleza de distribución global. En Chile, esta especie fue introducida tempranamente durante la colonización española, sin embargo, estudios previos basados en aloenzimas y fragmentos RAPD indican que las poblaciones del extremo sur de Chile estarían emparentadas con poblaciones provenientes de Inglaterra. En el presente estudio se analizó la variación geográfica de tres secuencias de cpSSR pertenecientes a 286 individuos de C. bursa-pastoris (ATCP46615, ATCP66701 y ATCP31017). Se recolectaron plantas de 14 poblaciones distribuidas en un amplio gradiente latitudinal en Chile continental. Sólo uno de los marcadores mostró variación (ATCP31017), lo que muestra la presencia de dos haplotipos de C. bursa-pastoris en el territorio. La distribución geográfica de estos haplotipos apoya la hipótesis que plantea que C. bursa-pastoris en Chile continental fue introducida al menos en dos oportunidades.

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We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from plant tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5-30 microg of total DNA from 200 mg of tissue fresh weight, depending on plant species and tissue source. It can be completed in as little as 5-6 h.

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Author and article information

Journal

Journal ID (publisher-id): gbot

Title: Gayana. Botánica

Abbreviated Title: Gayana Bot.

Publisher: Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción (Concepción, , Chile )

ISSN (Print): 0016-5301

ISSN (Electronic): 0717-6643

Publication date (Print and electronic): December 2014

Volume: 71

Issue: 2

Pages: 216-221

Affiliations

[04] Santiago orgnameONG Entorno Chile

[03] Santiago orgnameUniversidad Central de Chile orgdiv1Facultad de Arquitectura, Urbanismo y Paisaje orgdiv2Centro de Estudios Arquitectónicos, Urbanísticos y del Paisaje Chile

[01] Santiago orgnameUniversidad de Santiago de Chile orgdiv1Departamento de Biología orgdiv2Laboratorio de Ecología y Biodiversidad Vegetal Chile

[02] Santiago orgnameCentro para el Desarrollo de la Nanociencia y Nanotecnología Chile

Article

Publisher ID: S0717-66432014000200004 Publisher ID: S0717-6643(14)07100200004

DOI: 10.4067/S0717-66432014000200004

SO-VID: 3306a6de-9c44-481e-86a8-695750ff4315

License:

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

History

Date received : 11 September 2013

Date accepted : 17 June 2014

Page count

Figures: 0, Tables: 0, Equations: 0, References: 25, Pages: 6

Product

SciELO Chile

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