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      Presence of Bemisia tabaci (Hemiptera: Aleyrodidae) and Begomovirus, associated with tomato crops Solanum lycopersicum L. in Cundinamarca Translated title: Presencia de Bemisia tabaci (Hemiptera: Aleyrodidae) y Begomovirus, asociado al cultivo de tomate Solanum lycopersicum L. en Cundinamarca

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          Abstract

          This study was undertaken to determine the presence and distribution of species of whiteflies in Cundinamarca, in the tomato, located in nine municipalities; for this purpose, insect samples were collected from the lower third of leaflets to identify nymphs of the fourth instar and from the upper third to identify adults with molecular markers RAPD-PCR (primer OPA 04); we also analyzed the presence of Begomoviruses (Family Geminiviridae) in tomato plants with nucleic acid hybridization in the nylon membrane. The results indicated the presence of Trialeurodes vaporariorum (Westwood) as the predominant species at 40.9%, Bemisia tabaci biotype B at 4.5% and coexistence at 54.6%. B. tabaci biotype B had a wide distribution with respect to other studies, between 383 to 1,857 m a.s.l. The presence of Begomovirus was related to its vector B. tabaci biotype B in 4 out of 15 tomato-producing provinces of the department. The insecticides used for control are organophosphates (41.18%), neonicotenoids (29.41%), carbamates (17.65%) and pyrethroids (11.76%). We discuss the disturbing and rapid spread of B. tabaci biotype B in Cundinamarca

          Translated abstract

          Este trabajo se propuso determinar la presencia y distribución de las especies de moscas blancas en Cundinamarca, en cultivos de tomate de mesa ubicados en nueve municipios, con este fin se recolectaron muestras insectiles ubicadas en foliolos del tercio inferior, para identificar las ninfas del cuarto instar y del tercio superior para reconocer adultos mediante marcadores moleculares RAPD-PCR (cebador OPA 04); también se analizóla presencia de Begomovirus (Familia Geminiviridae) en plantas de tomate, bajo la técnica de hibridación de ácidos nucleídos en membrana de nylon. Los resultados indicaron la presencia de Trialeurodes vaporariorum (Westwood), como especie predominante en un 40,9%, Bemisia tabaci biotipo B en un 4,5% y en coexistencia en un 54,6%. Se presenta una amplia distribución, de B. tabaci biotipo B con respeto a otros estudios, con altitudes entre los 383 y 1.857 msnm. La presencia de Begomovirus está relacionada con su vector B. tabaci biotipo B en 4 de 15 provincias productoras de tomate del departamento. Los insecticidas empleados para su control, corresponden al grupo de los organofosforados (41,18%), neonicotenoides (29,41%), carbamatos (17.65%) y piretroides (11,76%). Se discute sobre la preocupante y rápida dispersión de B. tabaci biotipo B en Cundinamarca

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          Most cited references76

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          The Sweetpotato or Silverleaf Whiteflies: Biotypes of Bemisia tabaci or a Species Complex?

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            The Bemisia tabaci species complex

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              Plant viruses transmitted by whiteflies

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                Author and article information

                Journal
                agc
                Agronomía Colombiana
                Agron. colomb.
                Universidad Nacional de Colombia, Facultad de Agronomía (Bogotá, Distrito Capital, Colombia )
                0120-9965
                September 2012
                : 30
                : 3
                : 395-403
                Affiliations
                [02] orgnameInstituto Colombiano Agropecuario (ICA). everth.ebratt.ravelo@ 123456gmail.com
                [01] orgnameCorporación Universitaria Minuto de Dios orgdiv1Agroecological Engineering Program
                Article
                S0120-99652012000300011 S0120-9965(12)03000311
                31aadb69-8cd5-4d24-b53d-ec4b9afb8753

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 09 March 2011
                : 30 October 2012
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 46, Pages: 9
                Product

                SciELO Colombia

                Categories
                Protection of cultivations

                whiteflies,biotypes,vector,detection,RAPD-PCR,detección,biotipos,mosca blanca

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