Diarrheic toxins in field-sampled and cultivated Dinophysis spp. cells from southern Brazil

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Culture of Phytoplankton for Feeding Marine Invertebrates

Robert R. L. Guillard (1975)

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Determination of diarrhetic shellfish toxins in various dinoflagellate species

Jong Lee, Teruo Igarashi, Santiago Fraga … (1989)

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Isolation of pectenotoxin-2 from Dinophysis acuta and its conversion to pectenotoxin-2 seco acid, and preliminary assessment of their acute toxicities.

Christopher Miles, Shannon Briggs, Rex Munday … (2003)

We have developed a simple and effective method for isolating pectenotoxin-2 (PTX-2) from Dinophysis cells collected from a natural bloom. A two-step extraction procedure followed by two column chromatography steps produced PTX-2 in high purity suitable for use as an analytical standard and for toxicological studies. Incubation of purified PTX-2 with the supernatant from ultracentrifuged blue (Mytilus edulis) or Greenshell (Perna canaliculus) mussel hepatopancreas homogenate caused rapid conversion to pectenotoxin-2 seco acid (PTX-2 SA). Purification of PTX-2 SA was achieved by solvent extraction followed by column chromatography. PTX-2 and PTX-2 SA were fully characterized by LC-MS and NMR, and full (1)H and (13)C NMR assignments were obtained. Okadaic acid C(8)-diol ester was isolated during the purification of PTX-2, and its identity confirmed by NMR and LC-MS analyses. Pectenotoxin-2 seco acid methyl ester, identified by LC-MS, was also produced during the hydrolytic procedure due to the presence of methanol. PTX-2 was acutely toxic to mice by i.p. injection (LD(50)=219 microg/kg) but no effects were seen with PTX-2 SA at 5000 microg/kg. Neither PTX-2 nor PTX-2 SA was overtly toxic to mice by the oral route at doses up to 5000 microg/kg. No diarrhea was observed in mice dosed with either compound, suggesting that pectenotoxins do not belong in the diarrhetic shellfish poison group.

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Journal

Title: Journal of Applied Phycology

Abbreviated Title: J Appl Phycol

Publisher: Springer Nature

ISSN (Print): 0921-8971

ISSN (Electronic): 1573-5176

Publication date Created: August 2014

Publication date (Print): December 2013

Volume: 26

Issue: 4

Pages: 1727-1739

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DOI: 10.1007/s10811-013-0219-9

SO-VID: 2b283b40-d3e2-4657-95ef-59f1721b42dd

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Diarrheic toxins in field-sampled and cultivated Dinophysis spp. cells from southern Brazil

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Abstract

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SciELO Brazil

Most cited references 37

Culture of Phytoplankton for Feeding Marine Invertebrates

Determination of diarrhetic shellfish toxins in various dinoflagellate species

Isolation of pectenotoxin-2 from Dinophysis acuta and its conversion to pectenotoxin-2 seco acid, and preliminary assessment of their acute toxicities.

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Cited by 13

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