Mastomys natalensis and Lassa Fever, West Africa

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Abstract

PCR screening of 1,482 murid rodents from 13 genera caught in 18 different localities of Guinea, West Africa, showed Lassa virus infection only in molecularly typed Mastomys natalensis. Distribution of this rodent and relative abundance compared with M. erythroleucus correlates geographically with Lassa virus seroprevalence in humans.

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Most cited references 8

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A colchicine, hypotonic citrate, squash sequence for mammalian chromosomes.

Talitha C. Ford, J L Hamerton (1956)

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RT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene.

Stuart T. Nichol, Simon Vieth, Stephan Günther … (2007)

This study describes an RT-PCR assay targeting the L RNA segment of arenaviruses. Conserved regions were identified in the polymerase domain of the L gene on the basis of published sequences for Lassa virus, lymphocytic choriomeningitis virus (LCMV), Pichinde virus and Tacaribe virus, as well as 15 novel sequences for Lassa virus, LCMV, Ippy virus, Mobala virus and Mopeia virus determined in this study. Using these regions as target sites, a PCR assay for detection of all known Old World arenaviruses was developed and optimized. The concentration that yields 95% positive results in a set of replicate tests (95% detection limit) was determined to be 4290 copies of Lassa virus L RNA per ml of serum, corresponding to 30 copies per reaction. The ability of the assay to detect various Old World arenaviruses was demonstrated with in vitro transcribed RNA, material from infected cell cultures and samples from patients with Lassa fever and monkeys with LCMV-associated callitrichid hepatitis. The L gene PCR assay may be applicable: (i) as a complementary diagnostic test for Lassa virus and LCMV; (ii) to identify unknown Old World arenaviruses suspected as aetiological agents of disease; and (iii) for screening of potential reservoir hosts for unknown Old World arenaviruses.

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Lassa virus activity in Guinea: distribution of human antiviral antibody defined using enzyme-linked immunosorbent assay with recombinant antigen.

K Sidibe, Igor S. Lukashevich, Andrew J Clegg (1993)

More than 3,100 households in 27 selected villages distributed in the main geographic regions of Guinea were surveyed for the presence of Lassa virus-specific IgG antibodies (LVA), using an enzyme-linked immunosorbent assay (ELISA) with Lassa virus nucleocapsid protein expressed in insect cells infected with a recombinant baculovirus as antigen. The highest prevalence of LVA (25-55%) was found among inhabitants of tropical secondary forest (areas near Gueckedou, Yomou, and Lola) and guinea savannah (Faranah and Kindia areas), near the southern frontiers with Sierra Leone and Liberia. A much lower prevalence (4-7%) was found among inhabitants of mountainous (Pita, Labe, and Mali) and coastal (Boffa, Boké) areas. We found no discernible differences in LVA prevalence between males and females or among various age groups. Testing of 406 hospital staff members of the eight central hospitals in these areas for LVA revealed a similar distribution of seropositivity among hospitals in various prefectures. The highest prevalence of LVA in hospital staff (29-40%) was in the Gueckedou and Lola hospitals. Sera of LVA-positive persons were tested via Western blot analysis. Antibodies bound predominantly to NP and GP2 proteins.

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Author and article information

Journal

Journal ID (nlm-ta): Emerg Infect Dis

Journal ID (publisher-id): EID

Title: Emerging Infectious Diseases

Publisher: Centers for Disease Control and Prevention

ISSN (Print): 1080-6040

ISSN (Electronic): 1080-6059

Publication date (Print): December 2006

Volume: 12

Issue: 12

Pages: 1971-1974

Affiliations

[* ]Philipps University Institute of Virology, Marburg, Germany;

[† ]Muséum National d'Histoire Naturelle, Paris, France;

[‡ ]Washington University School of Medicine, Saint Louis, Missouri, USA;

[§ ]Projet de Recherches sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea;

[¶ ]Severtsov Institute of Ecology and Evolution, Moscow, Russia;

[# ]Institut Pasteur d'Abidjan, Abidjan, Côte d'Ivoire;

[** ]Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany;

[†† ]Leiden University Medical Center, Leiden, the Netherlands

Author notes

Address for correspondence: Emilie Lecompte, LEGS, CNRS, Avenue de la Terrasse, Bâtiment 13, BP 1, 91 198 Gif-sur-Yvette, France; email: emilie.lecompte@ 123456hotmail.fr

Article

Publisher ID: 06-0812

DOI: 10.3201/eid1212.060812

PMC ID: 3291371

PubMed ID: 17326956

SO-VID: 28404af0-4a30-411d-b20e-cbf71cd2f4d6

Mastomys natalensis and Lassa Fever, West Africa

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Abstract

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IET: COVID-19 related research

Most cited references 8

A colchicine, hypotonic citrate, squash sequence for mammalian chromosomes.

RT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene.

Lassa virus activity in Guinea: distribution of human antiviral antibody defined using enzyme-linked immunosorbent assay with recombinant antigen.

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