Network Analysis Indicates Microbial Assemblage Differences in Life Stages of Cladophora

Abstract

Cladophora represents a microscopic forest that provides many ecological niches fostering a diverse microbiota, with a complex and intimate relationship between Cladophora and bacteria. Many studies have focused on the microbiology of freshwater Cladophora , but the composition and succession of microorganisms in different life stages of Cladophora , especially in brackish water, have not been explored.

ABSTRACT

Cladophora represents a microscopic forest that provides many ecological niches and fosters a diverse microbiota. However, the microbial community on Cladophora in brackish lakes is still poorly understood. In this study, the epiphytic bacterial communities of Cladophora in Qinghai Lake were investigated at three life stages (attached, floating, and decomposing). We found that in the attached stage, Cladophora was enriched with chemoheterotrophic and aerobic microorganisms, including Yoonia - Loktanella and Granulosicoccus . The proportion of phototrophic bacteria was higher in the floating stage, especially Cyanobacteria . The decomposing stage fostered an abundance of bacteria that showed vertical heterogeneity from the surface to the bottom. The surface layer of Cladophora contained mainly stress-tolerant chemoheterotrophic and photoheterotrophic bacteria, including Porphyrobacter and Nonlabens . The microbial community in the middle layer was similar to that of floating-stage Cladophora . Purple oxidizing bacteria were enriched in the bottom layer, with Candidatus Chloroploca , Allochromatium , and Thiocapsa as the dominant genera. The Shannon and Chao1 indices of epibiotic bacterial communities increased monotonically from the attached stage to the decomposing stage. Microbial community composition and functional predictions indicate that a large number of sulfur cycle-associated bacteria play an important role in the development of Cladophora . These results suggest that the microbial assemblage on Cladophora in a brackish lake is complex and contributes to the cycling of materials.

IMPORTANCE Cladophora represents a microscopic forest that provides many ecological niches fostering a diverse microbiota, with a complex and intimate relationship between Cladophora and bacteria. Many studies have focused on the microbiology of freshwater Cladophora , but the composition and succession of microorganisms in different life stages of Cladophora , especially in brackish water, have not been explored. In this study, we investigated the microbial assemblages in the life stages of Cladophora in the brackish Qinghai Lake. We show that heterotrophic and photosynthetic autotrophic bacteria are enriched in attached and floating Cladophora , respectively, whereas the epiphytic bacterial community shows vertical heterogeneity in decomposing mats.