The aim of this study is to provide research performing organisations and research funding organisations (RPOs and RFOs) with practical advice on how to engage in an effective RRI institutionalisation. Therefore, we first looked at the most relevant drivers, challenges, and the most beneficial good practices potentially affecting RRI institutionalisation within RPOs and RFOs across Europe through a multi-step, multi-stakeholder consultation approach. The broad set of drivers, barriers and good practices identified at the consultation was methodologically divided into structural, cultural and interchange-related aspects. These aspects can theoretically exercise a positive or negative impact on the RRI institutionalisation, and their validity was tested in Living Labs by six organisations. By categorising these six implementers in terms of RRI readiness we were able to identify key factors and describe specific organisational circumstances conducive to a successful adoption and use of RRI principles and practices for three organisational types of RPOs/RFOs.