Correction: Prevalence Study and Genetic Typing of Bovine Viral Diarrhea Virus (BVDV) in Four Bovine Species in China

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The authors have prepared a revised manuscript to address comments left by readers regarding the species examined in this article as well as the classification of pestiviruses. Please view a revised manuscript provided as Supporting Information (S1 File), a revised Fig 2, and revised Supporting Information files S1 and S3 Tables here. 10.1371/journal.pone.0134777.g001 Fig 2 Phylogenetic analysis based on 5’-UTR (200 bp) and Npro (411 bp) sequences. A phylogenetic tree of the 5’-UTR was created using the nucleotide sequences of representative BVDV-1 isolates from each province and 37 reference strains retrieved from the GenBank database (S3 Table) (A); Phylogenetic tree analysis of the Npro gene was created using the nucleotide sequences of 11 selected BVDV-1 samples in this study and 32 BVDV reference strains retrieved from the GenBank database (B). ◆, isolates from this study; ◇, M31182 (JQ799141). The GenBank accession numbers of the reference strains used for Npro analysis were as follows: SD-1 (M96751), Osloss (M96687), VEDEVAC (AJ585412), 519 (AF144464), DeerNZ1 (U80903), Shitara0105 (AB359926), F-Au (AF287284), NCP03 (AB359927), 3186V6 (AF287282), J-Au (AF287286), W-Au (AF287290), A-Au (AF287283), L-Au (AF287287), G-Au (AF287285), 23–15 (AF287279), DeerGB1 (U80902), KS86-1ncp (AB078950), B440-06 (EU224257), TR-27 (EU163975), TR-29 (EU163977), ZM-95 (AF526381), Shitara0206 (AB359930), IS25CP01 (AB359931), BJ0703 (GU120261), HB-1 (KC695812), TR70 (KF154779), 2561 (JQ920343), UM/136/08 (LN515612), SI/207/12 (LN515611), NY-93 (AF502399), and Hobi (AY735486). Supporting Information S1 File A revised version of the article. (PDF) Click here for additional data file. S1 Table Proportion of samples tested by RT-PCR within each antibody category. (DOCX) Click here for additional data file. S3 Table 5’-UTR sequences of isolates and reference strains retrieved from GenBank. (DOCX) Click here for additional data file.

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Prevalence Study and Genetic Typing of Bovine Viral Diarrhea Virus (BVDV) in Four Bovine Species in China

Mingliang Deng, Sukun Ji, Wentao Fei … (2015)

To determine the nationwide status of persistent BVDV infection in different bovine species in China and compare different test methods, a total of 1379 serum samples from clinical healthy dairy cattle, beef cattle, yaks (Bos grunniens), and water buffalo (Bubalus bubalis) were collected in eight provinces of China from 2010 to 2013. The samples were analyzed using commercial antibody (Ab) and antigen (Ag) detection kits, and RT-PCR based on the 5’-UTR and Npro gene sequencing. Results showed that the overall positive rates for BVDV Ab, Ag and RT-PCR detection were 58.09% (801/1379), 1.39% (14/1010), and 22.64% (146/645), respectively, while the individual positive rates varied among regions, species, and farms. The average Ab-positive rates for dairy cattle, beef cattle, yaks, and water buffalo were 89.49% (298/333), 63.27% (248/392), 45.38% (236/520), and 14.18% (19/134), respectively, while the Ag-positive rates were 0.00% (0/116), 0.77% (3/392), 0.82% (3/368), and 5.97% (8/134), respectively, and the nucleic acid-positive rates detected by RT-PCR were 32.06% (42/131), 13.00% (26/200), 28.89% (52/180), and 19.40% (26/134), respectively. In addition, the RT-PCR products were sequenced and 124 5’-UTR sequences were obtained. Phylogenetic analysis of the 5’-UTR sequences indicated that all of the 124 BVDV-positive samples were BVDV-1 and subtyped into either BVDV-1b (33.06%), BVDV-1m (49.19%), or a new cluster, designated as BVDV-1u (17.74%). Phylogenetic analysis based on Npro sequences confirmed this novel subtype. In conclusion, this study revealed the prevalence of BVDV-1 in bovine species in China and the dominant subtypes. The high proportion of bovines with detectable viral nucleic acids in the sera, even in the presence of high Ab levels, revealed a serious threat to bovine health.

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Journal ID (nlm-ta): PLoS One

Journal ID (iso-abbrev): PLoS ONE

Journal ID (publisher-id): plos

Journal ID (pmc): plosone

Title: PLoS ONE

Publisher: Public Library of Science (San Francisco, CA USA )

ISSN (Electronic): 1932-6203

Publication date (Electronic): 31 July 2015

Publication date Collection: 2015

Volume: 10

Issue: 7

Electronic Location Identifier: e0134777

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Publisher ID: PONE-D-15-31496

DOI: 10.1371/journal.pone.0134777

PMC ID: 4521862

PubMed ID: 26230393

SO-VID: 187a130c-9c82-4c57-9fc3-030cb4ddbc2a

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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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