Isolation, Identification, and Molecular Characterization of Newcastle Disease Virus from Field Outbreaks in Chickens in Afghanistan.

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Abstract

Newcastle disease viruses (NDVs) in Afghanistan were isolated from three chicken farms and identified using a hemagglutination test and reverse transcription-polymerase chain reaction assay. Three isolates from each farm were sequenced to characterize the part of their fusion protein gene around the cleavage site. The characteristics of the fusion protein genes of the three isolates shown by phylogenic analysis indicated that the isolates were velogenic, belonged to the class II subgenotype VII 1.1, and were closely related to an identified Chinese NDV isolate. To our knowledge, this is the first time that NDV isolates from Afghanistan have been partially sequenced.

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Author and article information

Journal

Journal ID (iso-abbrev): Avian Dis

Title: Avian diseases

Publisher: American Association of Avian Pathologists (AAAP)

ISSN (Electronic): 1938-4351

ISSN (Print): 0005-2086

Publication date (Electronic): Jun 2022

Volume: 66

Issue: 2

Affiliations

[1 ] Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan.

[2 ] Laboratory of Microbiology, Department of Paraclinic, Faculty of Veterinary Sciences, Kabul University, Jamal Mina, Kabul 1006, Afghanistan.

[3 ] Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan.

[4 ] Bar-Ilan University, Begin-Sadat Center for Strategic Studies, Ramat Gan, 5290002, Israel.

[5 ] Laboratory of Animal Health, Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan, takehara@cc.tuat.ac.jp.