Proteome analysis of heart biopsies using a TRIzol-based protein extraction.

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

When dealing with rare samples of which only minute amounts are available, e.g. human heart tissue, simultaneous extraction of DNA, RNA, and proteins from the same sample is crucial for a comprehensive analysis on the physiological or pathological state of such precious tissue. In this study we provethe efficacy of a modified TRIzol protocol to extract proteins from samples of small size, such as endomyocardial biopsies (EMBs).

Related collections

Author and article information

Journal

Journal ID (iso-abbrev): Clin. Chim. Acta

Title: Clinica chimica acta; international journal of clinical chemistry

Publisher: Elsevier BV

ISSN (Electronic): 1873-3492

ISSN (Print): 0009-8981

Publication date (Electronic): Jan 01 2015

Volume: 438

Affiliations

[1 ] Department of Pharmacology, Physiology and Pathophysiology, Faculty of Pharmacy, University of Medicine and Pharmacy "Iuliu Hatieganu", Cluj-Napoca, Romania; Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany. Electronic address: pop.cristina@umfcluj.ro.

[2 ] Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany. Electronic address: amelings@uni-greifswald.de.

[3 ] Department of Internal Medicine B, University Medicine Greifswald, Germany. Electronic address: empen@uni-greifswald.de.

[4 ] Department of Internal Medicine B, University Medicine Greifswald, Germany. Electronic address: ruedebu@uni-greifswald.de.

[5 ] Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany. Electronic address: dhoplevm@uni-greifswald.de.

[6 ] Department of Internal Medicine B, University Medicine Greifswald, Germany; DZHK (German Center for Cardiovascular Research, Partner Site Greifswald), Germany. Electronic address: felix@uni-greifswald.de.

[7 ] Department of Toxicology, Faculty of Pharmacy, University of Medicine Pharmacy "Iuliu Hatieganu", Cluj-Napoca, Romania. Electronic address: floghin@umfcluj.ro.

[8 ] Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany; DZHK (German Center for Cardiovascular Research, Partner Site Greifswald), Germany. Electronic address: voelker@uni-greifswald.de.

[9 ] Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany; DZHK (German Center for Cardiovascular Research, Partner Site Greifswald), Germany. Electronic address: hammer@uni-greifswald.de.

Article

Publisher Item ID: S0009-8981(14)00385-4

DOI: 10.1016/j.cca.2014.08.033

PubMed ID: 25195007

SO-VID: 0029b7f1-6c2b-4b4c-85c1-903192add09d

History

Keywords: Protein extraction,Proteomics,TRIzol,2D PAGE,Heart biopsies,LC–MS/MS

Data availability:

Keywords: Protein extraction, Proteomics, TRIzol, 2D PAGE, Heart biopsies, LC–MS/MS

Proteome analysis of heart biopsies using a TRIzol-based protein extraction.

Read this article at

Abstract

Related collections

Genome Engineering using CRISPR

Author and article information

Journal

Affiliations

Article

History

Comments

Comment on this article

Similar content 209

Cited by 4