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      Normal background levels of air and surface mould reserve in English residential building stock: a preliminary study towards benchmarks based on NAHA measurements

      research-article
        1 , 2 , * , 3 , 2 , 4 , 2 , 1 , 2
      UCL Open Environment
      UCL Press
      mould, surface sampling, active (aggressive or activated) air sampling, NAHA, England, Denmark

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          Abstract

          This paper reports results obtained from a surface (both visually clean and dirty/dusty surfaces) and active (aggressive or activated) air testing scheme on 140 residential rooms in England, without visible water damage or mould growth, along with a few rooms with visible mould growth/water damage tested for comparison purposes. The aim was to establish normal background levels of mould in non-water-damaged interiors to benchmark a ‘normal’ indoor environment, and in turn when there is a need for further investigation, and, possibly, remediation. Air and surface mould was quantified based on the activity of β-N-acetylhexosaminidase (EC 3.2.1.52; NAHA). The obtained readings showed a log-normal distribution. Ninety-eight percent of the samples obtained from visually clean surfaces were equal to or less than 25 relative fluorescence units (RFU), which is suggested to be the higher bound for the range which can be used as a success criterion for surface cleaning/remediation. Of samples obtained from visually dirty/dusty surfaces, around 98% were below 450 RFU, which is suggested to define the lower-bound for abnormally high levels of mould, rare even on dirty/dusty surfaces. Similarly, around 98% of the air samples were found to have 1700 RFU or below. Values above 1700 RFU are therefore deemed unlikely in a non-problem indoor environment and can be indicative of a possible problem inducing mould growth. The samples with values below 1700 were further divided into three proposed sub-categories. Finally, the obtained RFU values and the suggested benchmarks were compared to those obtained from 17 non-residential indoor environments tested previously in Copenhagen, and the benchmarks that are currently used in Danish national standards, and they were both found to be highly congruent, suggesting that local climate regimes and room functions might not be as influential on indoor mould levels as commonly thought, or that the nuances between England and Denmark in terms of these factors are not strong enough to lead to sizable changes in the typical indoor mould levels in these countries’ building stocks.

          Most cited references42

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          Fuel poverty and human health: A review of recent evidence

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            Indoor fungal composition is geographically patterned and more diverse in temperate zones than in the tropics.

            Fungi are ubiquitous components of indoor human environments, where most contact between humans and microbes occurs. The majority of these organisms apparently play a neutral role, but some are detrimental to human lifestyles and health. Recent studies that used culture-independent sampling methods demonstrated a high diversity of indoor fungi distinct from that of outdoor environments. Others have shown temporal fluctuations of fungal assemblages in human environments and modest correlations with human activity, but global-scale patterns have not been examined, despite the manifest significance of biogeography in other microbial systems. Here we present a global survey of fungi from indoor environments (n = 72), using both taxonomic and phylogeny-informative molecular markers to determine whether global or local indoor factors determine indoor fungal composition. Contrary to common ecological patterns, we show that fungal diversity is significantly higher in temperate zones than in the tropics, with distance from the equator being the best predictor of phylogenetic community similarity. Fungal composition is significantly auto-correlated at the national and hemispheric spatial scales. Remarkably, building function has no significant effect on indoor fungal composition, despite stark contrasts between architecture and materials of some buildings in close proximity. Distribution of individual taxa is significantly range- and latitude-limited compared with a null model of randomized distribution. Our results suggest that factors driving fungal composition are primarily global rather than mediated by building design or function.
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              Polybrominated diphenyl ethers in relation to autism and developmental delay: a case-control study

              Background Polybrominated diphenyl ethers (PBDEs) are flame retardants used widely and in increasing amounts in the U.S. over the last few decades. PBDEs and their metabolites cross the placenta and studies in rodents demonstrate neurodevelopmental toxicity from prenatal exposures. PBDE exposures occur both via breastfeeding and hand-to-mouth activities in small children. Methods Participants were 100 children from the CHARGE ( CH ildhood A utism R isk from G enetics and the E nvironment) Study, a case-control epidemiologic investigation of children with autism/autism spectrum disorder, with developmental delay and from the general population. Diagnoses of autism were confirmed by the Autism Diagnostic Observation Schedule and Autism Diagnostic Inventory-Revised, and of developmental delay using the Mullen's Scales of Early Learning and the Vineland Adaptive Behavior Scales. Typically developing controls were those with no evidence of delay, autism, or autism spectrum disorder. Eleven PBDE congeners were measured by gas chromatography/mass spectrometry from serum specimens collected after children were assessed. Logistic regression was used to evaluate the association between plasma PBDEs and autism. Results Children with autism/autism spectrum disorder and developmental delay were similar to typically developing controls for all PBDE congeners, but levels were high for all three groups. Conclusions Plasma samples collected post-diagnosis in this study may not represent early life exposures due to changes in diet and introduction of new household products containing PBDEs. Studies with direct measurements of prenatal or infant exposures are needed to assess the possible causal role for these compounds in autism spectrum disorders.
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                Author and article information

                Journal
                UCL Open Environ
                UCLOE
                UCL Open Environment
                UCL Open Environ
                UCL Press (UK )
                2632-0886
                06 March 2020
                2020
                : 2
                : e005
                Affiliations
                [1 ]University College London (UCL), Department of Civil, Environmental and Geomatic Engineering (CEGE), Epicentre Research Group, London WC1E 6DE, UK
                [2 ]UK Centre for Moisture in Buildings (UKCMB), University College London, London WC1H 0NN, UK
                [3 ]Mycometer A/S, Dr Neergaards Vej 3, 2970 Hørsholm, Denmark
                [4 ]University College London (UCL), Institute of Environmental Design and Engineering (IEDE), London WC1H 0NN, UK
                Author notes
                *Corresponding author: Email: y.aktas@ 123456ucl.ac.uk
                Author information
                https://orcid.org/0000-0002-6317-2673
                https://orcid.org/0000-0001-9398-3066
                https://orcid.org/0000-0003-3864-2052
                Article
                10.14324/111.444/ucloe.000005
                0ab99ea5-fc36-4eb2-bc8f-d040978f491d
                © 2020 The Authors.

                This is an open access article distributed under the terms of the Creative Commons Attribution Licence (CC BY) 4.0, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

                History
                : 10 September 2019
                : 13 February 2020
                Page count
                Figures: 3, References: 38, Pages: 9
                Categories
                Research Article

                mould,surface sampling,active (aggressive or activated) air sampling,NAHA,England,Denmark

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