Differentiation of Aggregating Brain Cell Cultures

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Author(s): Nicholas W. Seeds

Publication date (Print): 1973

Publisher: Springer US

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Most cited references 57

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Observations on the living developing nerve fiber

R. G. Harrison (1906)

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A protein binding assay for adenosine 3':5'-cyclic monophosphate.

Morgan Gilman (1970)

A simple and sensitive assay for adenosine 3':5'-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10(-9) M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.