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Understanding Light Microscopy
monograph
Author(s):
Jeremy Sanderson
Publication date
(Online):
March 13 2019
Publisher:
Wiley
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Author and book information
Book
ISBN (Print):
9780470973752
ISBN (Electronic):
9781118696736
Publication date (Print):
April 24 2019
Publication date (Online):
March 13 2019
DOI:
10.1002/9781118696736
SO-VID:
59ee1ec5-e06b-42b9-a2a5-0e926593d233
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Book chapters
pp. i
Front Matter
pp. 1
Our Eyes and the Microscope
pp. 29
Light
pp. 55
Basic Microscope Optics
pp. 75
Microscope Anatomy and Design
pp. 91
Ergonomics
pp. 101
Optical Aberrations of the Microscope
pp. 127
The Microscope Objective
pp. 161
Condensers and Eyepieces
pp. 177
Illumination in the Microscope
pp. 211
Diffraction and Image Formation in Microscopy
pp. 243
Contrast Generation and Enhancement
pp. 289
Reflected-Light Microscopy
pp. 317
Polarised-Light Microscopy: Part 1 - Theory
pp. 347
Polarised-Light Microscopy: Part 2 - Applied
pp. 383
Fluorescence Microscopy
pp. 405
Fluorophores and Fluorescent Proteins
pp. 425
Optical Sectioning and Confocal Microscopy
pp. 447
Operating the Confocal Microscope
pp. 483
Light-Sheet Microscopy
pp. 507
Bleed-Through and Spectral Unmixing
pp. 523
Deconvolution
pp. 543
Multi-Photon Microscopy
pp. 561
Total Internal Reflection Fluorescence Microscopy
pp. 569
FRAP and FRET
pp. 587
Colocalisation
pp. 613
Super-Resolution Fluorescence Microscopy
pp. 637
Choosing a Microscope Platform and Core Imaging Facilities
pp. 663
Biological Specimen Preparation
pp. 687
Materials Specimen Preparation
pp. 707
Recording the Image: Part 1 - Theory
pp. 733
Recording the Image: Part 2 - Applied
pp. 769
Buying, and Tendering for, a Light Microscope
pp. 773
Troubleshooting Poor Image Quality
pp. 775
The Michel-Lévy Interference Colour Chart
pp. 779
Cleaning and Maintenance of the Light Microscope
pp. 783
Selected Suppliers
pp. 787
Historical Background
pp. 791
Timeline of Key Events
pp. 799
Index
pp. 816
Foldout
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