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      Neuroanatomical Tract-Tracing Methods 

      Experimental Neuroanatomy

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      Springer US

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          An autoradiographic study of the organization of intrahippocampal association pathways in the rat.

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            Projection of the lateral part of the entorhinal area to the hippocampus and fascia dentata.

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              An improved Timm sulphide silver method for light and electron microscopic localization of heavy metals in biological tissues.

              Modifications of the Timm sulphide silver method for the demonstration of heavy metals are described. To improve the structural preservation of the tissues perfusion with a glutaraldehyde fixature is employed before perfusion with the sodium sulphide solution. For the subsequent staining for light and electron microscopy, procedures for plastic embedding, paraffin embedding and cryostat sectioning are presented. Examples from several tissues are shown, including the pituitary, pancreas, intestine, tongue, kidney, testis and brain. The staining of autolytic, postmortal human brain tissue is demonstrated.
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                1981
                : 1-53
                10.1007/978-1-4613-3189-6_1
                245ba36a-e2e0-4c66-93f4-4dd83cc5ae4a
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